碲化镉量子点引发的蛋白质电晕加剧了巨噬细胞的炎症反应

IF 4.7 3区 环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES
Na Liu , Ying Liang , Tingting Wei , Xiaoquan Huang , Ting Zhang , Meng Tang
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引用次数: 0

摘要

纳米生物界面是纳米医学中的一个重要问题。当纳米粒子(NPs)与细胞接触时,它们会与蛋白质形成称为蛋白质电晕(PC)的复合物。碲化镉量子点(CdTe QDs)已被用作生物成像探针和巨噬细胞热敏剂。然而,蛋白质电晕对碲化镉量子点行为的影响还不甚了解。巨噬细胞在抵御 NPs 方面起着至关重要的作用。本研究使用 RAW264.7 细胞研究了在胎牛血清中形成 PC 之前和之后暴露于碲化镉 QDs 时巨噬细胞的炎症反应。结果表明,蛋白电晕将更多的巨噬细胞极化为 M1 表型。转录组学分析表明,与 1.0 μM 的碲化镉 QDs 相比,PC-碲化镉 QDs 改变了巨噬细胞中更多的差异表达基因(DEGs)(177 个和 398 个)。受 PC-CdTe QDs 影响的 DEGs 包含多种个性化炎症细胞因子。PC形成后富集的通路包括细胞因子-细胞因子受体相互作用、NOD样受体信号通路和TNF信号通路等。此外,PC 特异性地加剧了 CCL2 和 IL-1β 蛋白的过度表达。重要的是,PC 改变了碲化镉 QD 诱导的热蛋白沉积机制,使其从激活 NLRC4 转变为同时激活 NLRP1 和 NLRP3 炎症体,从裂解 GSDMD 和 GSDMB 转变为仅裂解 GSDMB。总之,蛋白电晕加剧了碲化镉 QDs 在巨噬细胞中诱导的炎症反应。这项研究为了解蛋白电晕对碲化镉 QDs 的促炎症效应提供了宝贵的见解,并通过利用或消除这种生物界面效应,对其在生物成像或巨噬细胞热诊断中的应用产生了影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Protein corona exacerbated inflammatory response in macrophages elicited by CdTe quantum dots

Protein corona exacerbated inflammatory response in macrophages elicited by CdTe quantum dots

Protein corona exacerbated inflammatory response in macrophages elicited by CdTe quantum dots

Nano-bio interface is significant concern in nanomedicine. When nanoparticles (NPs) come into contact with cells, they form complexes with proteins known as protein corona (PC). Cadmium telluride quantum dots (CdTe QDs) have been applied as bioimaging probes and for macrophage theragnostic. However, the impact of protein corona on the behavior of CdTe QDs is not well understood. Macrophages play a crucial role in defending against NPs. In this study, RAW264.7 cells were used to investigated the inflammatory response in macrophages when exposed to CdTe QDs before and after PC formation in fetal bovine serum. The results indicated that protein corona polarized more macrophages towards M1 phenotype. Transcriptomics analysis revealed that PC-CdTe QDs altered a greater number of differentially expressed genes (DEGs) compared to CdTe QDs (177 and 398) at 1.0 μM in macrophages. The DEGs affected by PC-CdTe QDs contained several personalized inflammatory cytokines. The enriched pathways after PC formation included Cytokine-cytokine receptor interaction, NOD-like receptor signaling pathway, and TNF signaling pathway, etc. Furthermore, PC specifically exacerbated the overexpression of CCL2 and IL-1β proteins. Importantly, PC altered the mechanism of CdTe QD-induced pyroptosis, shifting it from activating NLRC4 to both NLRP1 and NLRP3 inflammasomes, and from cleaving GSDMD and GSDMB to GSDMB alone. Overall, protein corona exacerbated the inflammatory response induced by CdTe QDs in macrophages. This study provides valuable insight into the pro-inflammatory effect of protein corona on CdTe QDs, with implications for their use in bioimaging or macrophage theragnostic by either exploiting or eliminating this biological interface effect.

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来源期刊
NanoImpact
NanoImpact Social Sciences-Safety Research
CiteScore
11.00
自引率
6.10%
发文量
69
审稿时长
23 days
期刊介绍: NanoImpact is a multidisciplinary journal that focuses on nanosafety research and areas related to the impacts of manufactured nanomaterials on human and environmental systems and the behavior of nanomaterials in these systems.
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