C. Deepika , S.R. Venkatachalam , A. Yuvaraja , P. Arutchenthil , N. Indra , V. Ravichandran , P. Veeramani , P. Kathirvelan
{"title":"蓖麻雌花品系的评价及蓖麻（Ricinus communis L.）抗镰刀菌枯萎病遗传模式的分析","authors":"C. Deepika , S.R. Venkatachalam , A. Yuvaraja , P. Arutchenthil , N. Indra , V. Ravichandran , P. Veeramani , P. Kathirvelan","doi":"10.1016/j.cpb.2024.100319","DOIUrl":null,"url":null,"abstract":"<div><p>Castor is an industrially economic and valuable oilseed crop cultivated worldwide. There is a constant upsurge in demand for its oil. But wilt caused by <em>Fusarium spp.</em> is a devastating disease that severely affects the productivity depending upon the crop stage. Stable high yielding pistillate lines serve as donors in heterosis breeding programme and for further biotechnological advancements. Genetic characterization of wilt resistance indicated the role of duplicate dominant epistasis in YTP 1 × TMV 5, complementary epistasis in DPC 9 × JP 65 and JP 65 × SKI 215, duplicate recessive epistasis in YRCP 1 × DPC 9. Monogenic recessive nature of wilt resistance was reported in other four cross combinations <em>viz.,</em> YRCP 2 × JP 65, SKP 84 × JP 65, YRCP 2 × DPC 9 and YRCP 2 × SKP 84. Magnified images taken using LED phase contrast microscope portrayed the presence of microconidia and macroconidia and Scanning Electron Microscope (SEM) image analysis showed the presence of intact internal cell structures in resistant check (48−1) while the cell structures were disturbed with mycelial growth in the susceptible check (JI 35). Among 21 pistillate lines screened, seven <em>viz.,</em> DPC 9, DPC 16, SKP 84, JP 96, GEETA, M 574 and M 619–1 were resistant. By screening P1, P2, F1, F2, BC<sub>1</sub>F<sub>1</sub> (P1) and BC<sub>1</sub>F<sub>1</sub> (P2) generations of eight crosses under field and pot test method, the stable pistillate line DPC 9 was found to be wilt resistant. F<sub>1</sub> generation plants expressed 100% susceptibility indicating the recessive nature of wilt resistance. JP 65 × SKI 215 and YRCP 1 × DPC 9 showed the minimum incidence comparing other F<sub>2</sub> populations. The backcross (YRCP 1 × DPC 9) × DPC 9 was found to possess the lowest wilt incidence compared to other populations under field and green house condition. Hence the identified lines could be better used to develop wilt resistant high yielding hybrid and for further identifying and introgressing genomic regions conferring wilt resistance to high yielding popular variety through linkage/QTL mapping technique.</p></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":"37 ","pages":"Article 100319"},"PeriodicalIF":5.4000,"publicationDate":"2024-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S221466282400001X/pdfft?md5=c115dcb85a0496f6a6bd8a0b3dca03c3&pid=1-s2.0-S221466282400001X-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Evaluation of castor pistillate lines and analysis of mode of inheritance for resistance to Fusarium wilt disease in castor (Ricinus communis L.)\",\"authors\":\"C. Deepika , S.R. Venkatachalam , A. Yuvaraja , P. Arutchenthil , N. Indra , V. Ravichandran , P. Veeramani , P. Kathirvelan\",\"doi\":\"10.1016/j.cpb.2024.100319\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Castor is an industrially economic and valuable oilseed crop cultivated worldwide. There is a constant upsurge in demand for its oil. But wilt caused by <em>Fusarium spp.</em> is a devastating disease that severely affects the productivity depending upon the crop stage. Stable high yielding pistillate lines serve as donors in heterosis breeding programme and for further biotechnological advancements. Genetic characterization of wilt resistance indicated the role of duplicate dominant epistasis in YTP 1 × TMV 5, complementary epistasis in DPC 9 × JP 65 and JP 65 × SKI 215, duplicate recessive epistasis in YRCP 1 × DPC 9. Monogenic recessive nature of wilt resistance was reported in other four cross combinations <em>viz.,</em> YRCP 2 × JP 65, SKP 84 × JP 65, YRCP 2 × DPC 9 and YRCP 2 × SKP 84. Magnified images taken using LED phase contrast microscope portrayed the presence of microconidia and macroconidia and Scanning Electron Microscope (SEM) image analysis showed the presence of intact internal cell structures in resistant check (48−1) while the cell structures were disturbed with mycelial growth in the susceptible check (JI 35). Among 21 pistillate lines screened, seven <em>viz.,</em> DPC 9, DPC 16, SKP 84, JP 96, GEETA, M 574 and M 619–1 were resistant. By screening P1, P2, F1, F2, BC<sub>1</sub>F<sub>1</sub> (P1) and BC<sub>1</sub>F<sub>1</sub> (P2) generations of eight crosses under field and pot test method, the stable pistillate line DPC 9 was found to be wilt resistant. F<sub>1</sub> generation plants expressed 100% susceptibility indicating the recessive nature of wilt resistance. JP 65 × SKI 215 and YRCP 1 × DPC 9 showed the minimum incidence comparing other F<sub>2</sub> populations. The backcross (YRCP 1 × DPC 9) × DPC 9 was found to possess the lowest wilt incidence compared to other populations under field and green house condition. 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Evaluation of castor pistillate lines and analysis of mode of inheritance for resistance to Fusarium wilt disease in castor (Ricinus communis L.)
Castor is an industrially economic and valuable oilseed crop cultivated worldwide. There is a constant upsurge in demand for its oil. But wilt caused by Fusarium spp. is a devastating disease that severely affects the productivity depending upon the crop stage. Stable high yielding pistillate lines serve as donors in heterosis breeding programme and for further biotechnological advancements. Genetic characterization of wilt resistance indicated the role of duplicate dominant epistasis in YTP 1 × TMV 5, complementary epistasis in DPC 9 × JP 65 and JP 65 × SKI 215, duplicate recessive epistasis in YRCP 1 × DPC 9. Monogenic recessive nature of wilt resistance was reported in other four cross combinations viz., YRCP 2 × JP 65, SKP 84 × JP 65, YRCP 2 × DPC 9 and YRCP 2 × SKP 84. Magnified images taken using LED phase contrast microscope portrayed the presence of microconidia and macroconidia and Scanning Electron Microscope (SEM) image analysis showed the presence of intact internal cell structures in resistant check (48−1) while the cell structures were disturbed with mycelial growth in the susceptible check (JI 35). Among 21 pistillate lines screened, seven viz., DPC 9, DPC 16, SKP 84, JP 96, GEETA, M 574 and M 619–1 were resistant. By screening P1, P2, F1, F2, BC1F1 (P1) and BC1F1 (P2) generations of eight crosses under field and pot test method, the stable pistillate line DPC 9 was found to be wilt resistant. F1 generation plants expressed 100% susceptibility indicating the recessive nature of wilt resistance. JP 65 × SKI 215 and YRCP 1 × DPC 9 showed the minimum incidence comparing other F2 populations. The backcross (YRCP 1 × DPC 9) × DPC 9 was found to possess the lowest wilt incidence compared to other populations under field and green house condition. Hence the identified lines could be better used to develop wilt resistant high yielding hybrid and for further identifying and introgressing genomic regions conferring wilt resistance to high yielding popular variety through linkage/QTL mapping technique.
期刊介绍：
Current Plant Biology aims to acknowledge and encourage interdisciplinary research in fundamental plant sciences with scope to address crop improvement, biodiversity, nutrition and human health. It publishes review articles, original research papers, method papers and short articles in plant research fields, such as systems biology, cell biology, genetics, epigenetics, mathematical modeling, signal transduction, plant-microbe interactions, synthetic biology, developmental biology, biochemistry, molecular biology, physiology, biotechnologies, bioinformatics and plant genomic resources.
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