根据全基因组测序鉴定从家庭成员中分离出的百日咳博德特氏菌的菌株特征。

IF 1.9 4区医学 Q4 IMMUNOLOGY

Microbiology and Immunology Pub Date : 2024-01-15 DOI:10.1111/1348-0421.13113

Kazunari Kamachi, Kentaro Koide, Nao Otsuka, Masataka Goto, Tsuyoshi Kenri

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引用次数: 0

摘要

我们通过全基因组测序评估了百日咳病原体百日咳博德特氏菌在家庭中的遗传多样性。在对从 11 个家庭收集的 23 个分离株进行配对比较时，单核苷酸多态性（SNP）分析表明分离株配对之间的 SNP 多样性极低（≤1 个 SNP）：10 个家庭未检测到 SNP，其余家庭检测到一个 SNP。这个 SNP 在百日咳杆菌中并不常见，它导致烟酸磷酸核糖转移酶中的一个非同义替换（Ala303Thr）。我们证明，同一菌株会在家庭成员之间传播，百日咳杆菌在家庭传播过程中基因组稳定。

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Strain identity of Bordetella pertussis isolates from household members based on whole-genome sequencing

We evaluated the genetic diversity of Bordetella pertussis, the causative agent of pertussis, within households by whole-genome sequencing. In pairwise comparisons of 23 isolates collected from 11 households, single-nucleotide polymorphism (SNP) analysis revealed extremely low SNP diversity (≤1 SNP) between isolate pairs: no SNPs were detected in 10 households and one SNP was obtained in the remaining household. This SNP was uncommon for B. pertussis and resulted in a nonsynonymous substitution (Ala303Thr) in nicotinate phosphoribosyltransferase. We demonstrated that the same strain is transmitted between household members and that B. pertussis is genomically stable during household transmission.

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来源期刊

Microbiology and Immunology 医学-免疫学

CiteScore

5.20

自引率

3.80%

发文量

审稿时长

1 months

期刊介绍： Microbiology and Immunology is published in association with Japanese Society for Bacteriology, Japanese Society for Virology, and Japanese Society for Host Defense Research. It is peer-reviewed publication that provides insight into the study of microbes and the host immune, biological and physiological responses. Fields covered by Microbiology and Immunology include:Bacteriology|Virology|Immunology|pathogenic infections in human, animals and plants|pathogenicity and virulence factors such as microbial toxins and cell-surface components|factors involved in host defense, inflammation, development of vaccines|antimicrobial agents and drug resistance of microbes|genomics and proteomics.