人类内脏利什曼病疗效的分子监测。

IF 1.9 4区 医学 Q3 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH
Sutopa Roy, Srija Moulik, Surya Jyati Chaudhuri, Manab K Ghosh, R P Goswami, Bibhuti Saha, Mitali Chatterjee
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引用次数: 0

摘要

背景:消除内脏利什曼病计划的集中努力已导致病例急剧下降,目前的挑战是疾病监测,本研究旨在对此进行评估:消除内脏利什曼病计划的集中努力已导致病例急剧下降,目前的挑战是疾病监测,本研究旨在对此进行评估:方法:利用利什曼原虫靶向 qPCR 对发病时和治疗结束后(49 例)的寄生虫量进行量化;治疗结束后对另外 80 例病例进行监测:结果:发病时的寄生虫量为 13 461.00 (2560.00-37764.00)/µg gDNA,完成治疗后,49 例中有 47 例的寄生虫量减少到 1(1-1)/µg gDNA,治疗后 2 个月,除 80 例中有 6 例 qPCR 阳性外,其他病例的寄生虫量同样减少到 1(1-1)/µg gDNA:结论:在 129 例内脏利什曼病病例中,通过量化寄生虫负荷进行 qPCR 检测可有效监测治疗效果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Molecular monitoring of treatment efficacy in human visceral leishmaniasis.

Background: Focused efforts of the visceral leishmaniasis elimination program have led to a drastic decline in cases, and the present challenge is disease monitoring, which this study aimed to assess.

Methods: A Leishmania kinetoplastid-targeted qPCR quantified parasite load at disease presentation, and following treatment completion (n=49); an additional 80 cases were monitored after completion of treatment.

Results: The parasite load at disease presentation was 13 461.00 (2560.00-37764.00)/µg gDNA, which upon completion of treatment reduced in 47 of 49 cases to 1(1-1)/µg gDNA, p<0.0001. In 80 cases that presented >2 months post-treatment, their parasite burden similarly decreased to 1(1-1)/µg gDNA except in 6 of 80 cases, which were qPCR positive.

Conclusion: In 129 cases of visceral leishmaniasis, qPCR by quantification of parasite burden proved effective for monitoring treatment.

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来源期刊
Transactions of The Royal Society of Tropical Medicine and Hygiene
Transactions of The Royal Society of Tropical Medicine and Hygiene 医学-公共卫生、环境卫生与职业卫生
CiteScore
4.00
自引率
9.10%
发文量
115
审稿时长
4-8 weeks
期刊介绍: Transactions of the Royal Society of Tropical Medicine and Hygiene publishes authoritative and impactful original, peer-reviewed articles and reviews on all aspects of tropical medicine.
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