木薯（Manihot esculenta）虫害 Ferrisia virgata（同翅目：褐球虫科：假球虫科）相关内生细菌的分子鉴定

IF 0.6 Q4 ENVIRONMENTAL SCIENCES

Journal of environmental biology Pub Date : 2024-01-02 DOI:10.22438/jeb/45/1/mrn-5170

B.G. Sangeetha, P. Drishya

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引用次数: 0

摘要

目的：鉴定从木薯植物中采集的蚧壳虫样本，并鉴定与蚧壳虫相关的内生细菌：使用线粒体 C y t o c h r o m e o x i d a s e ( C O X - 1 ) C 1 - J - 2 1 8 3 F(CAACATTTATTTTGATTTTTTGG) 和 CI-N-2568R (GCWACWACRTAATAKGTATCATG) 引物对蚧壳虫进行分子鉴定。使用 16S rRNA 基因通用引物对细菌进行分子鉴定：蚧壳虫被鉴定为F. virgata，与蚧壳虫相关的内生菌被鉴定为Lysinibacillus fusiformis和Bacillus cereus。解释：与蚧壳虫相关的内生菌在完成寄主昆虫的生命周期和为寄主昆虫提供营养方面发挥了重要作用。关键字木薯内源共生菌铁锈蚧

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Molecular identification of endosymbiotic bacteria associated with Ferrisia virgata (Homoptera: Coccoidea: Pseudococcidae) infesting cassava (Manihot esculenta)

Aim: To identify the mealy bug samples collected from cassava plants and identification of endosymbiotic bacteria associated with the mealy bugs Methodology: Molecular identification of mealy bugs was done using mitochondrial c y t o c h r o m e o x i d a s e ( C O X - 1 ) C 1 - J - 2 1 8 3 F(CAACATTTATTTTGATTTTTTGG) and CI-N-2568R (GCWACWACRTAATAKGTATCATG) primers. The molecular identification of bacteria was done using 16S rRNA gene universal primers Results: The mealy bug was identified as F. virgata and the endosymbionts associated with mealy bugs were identified as Lysinibacillus fusiformis and Bacillus cereus. Interpretation: The endosymbionts associated with the mealy bugs play significant role in completing lifecycle of insects host and for providing nutrients to the host insects. Key words: Cassava, Endosymbionts, Ferissia virgata, Mealybug

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