蛋白质吸附的原位傅立叶变换红外全反射光谱和模型研究:二氧化钛锐钛矿上的膨胀牛血清白蛋白案例。

IF 1.6 4区 医学 Q4 BIOPHYSICS
Biointerphases Pub Date : 2024-01-01 DOI:10.1116/6.0003020
Moustafa Tadjine, Fatima Bouzidi, Abderrezak Berbri, Hamid Nehmar, Ahmed Bouhekka
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引用次数: 0

摘要

本实验和建模研究的目的是研究 pH 值效应,并分别确定牛血清白蛋白(BSA)在二氧化钛锐钛矿表面的表面覆盖率和吸附常数(Ka)。在流动池中使用原位傅立叶变换红外-衰减全反射光谱来研究 BSA 在多孔二氧化钛锐钛矿薄膜上的吸附情况。实验在不同 pH 值、浓度为 10-6 mol/l 的水溶液中进行。从理论上讲,我们扩展了基于耦合微分方程系统的双态模型,增加了一个解吸参数 Kd2,以表示未折叠状态。模型的求解考虑了吸附系数(Ka)、解吸系数(Kd1,2)、转化系数(Kf)以及溶液中蛋白质的初始浓度(C0)。研究结果清楚地表明,溶液的 pH 值极大地改变了 BSA 的吸附行为,而数学分析方案则使我们能够确定原生态 (θ1)、展开态 (θ2)和完全态 (θ)的表面覆盖率。最后,在 pH = 1.7 的条件下,将近似模型很好地应用于吸附在锐钛矿二氧化钛上的膨胀 BSA 的实验工作,结果表明 Ka 值 = (408.36 ± 0.996) × 102 mol-1 l min-1。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
In situ Fourier transform infrared-attenuated total reflection spectroscopy and modeling investigation of protein adsorption: Case of expanded bovine serum albumin on titanium dioxide anatase.

The purpose of this experimental and modeling research is to study the pH effect and to determine the surface coverage plus the adsorption constant (Ka) of bovine serum albumin (BSA) protein adsorbed on TiO2 anatase surface, respectively. In situ Fourier transform infrared-attenuated total reflection spectroscopy in a flow-through cell was used to study the BSA adsorption on porous TiO2 anatase films. The experiments were performed in water solution, under different pH values, at a concentration of 10-6 mol/l. Theoretically, we extended the two-state model, based on a system of coupled differential equations, by adding a desorption parameter Kd2, for unfolded state. The model was solved taking into account the adsorption (Ka), desorption (Kd1,2), transformation (Kf) coefficients, and the initial solution protein concentration (C0). The findings clearly illustrated that the solution pH drastically changed the behavior of BSA adsorption, whereas the mathematical analytical solutions allowed us to determine the native state (θ1), the unfolded state (θ2), and the full one (θ) surface coverages. Finally, a good application of the approximated model on the experimental work, expanded BSA adsorbed on TiO2 anatase at pH = 1.7, indicated a value of Ka = (408.36 ± 0.996) × 102 mol-1 l min-1.

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来源期刊
Biointerphases
Biointerphases 生物-材料科学:生物材料
自引率
0.00%
发文量
35
期刊介绍: Biointerphases emphasizes quantitative characterization of biomaterials and biological interfaces. As an interdisciplinary journal, a strong foundation of chemistry, physics, biology, engineering, theory, and/or modelling is incorporated into originated articles, reviews, and opinionated essays. In addition to regular submissions, the journal regularly features In Focus sections, targeted on specific topics and edited by experts in the field. Biointerphases is an international journal with excellence in scientific peer-review. Biointerphases is indexed in PubMed and the Science Citation Index (Clarivate Analytics). Accepted papers appear online immediately after proof processing and are uploaded to key citation sources daily. The journal is based on a mixed subscription and open-access model: Typically, authors can publish without any page charges but if the authors wish to publish open access, they can do so for a modest fee. Topics include: bio-surface modification nano-bio interface protein-surface interactions cell-surface interactions in vivo and in vitro systems biofilms / biofouling biosensors / biodiagnostics bio on a chip coatings interface spectroscopy biotribology / biorheology molecular recognition ambient diagnostic methods interface modelling adhesion phenomena.
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