Pietro G Signorile, Sabrina Dominici, Rosa Viceconte, Alfonso Baldi
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Prototype of a salivary assay for quantification of two biomarkers for in vitro diagnosis of endometriosis
Endometriosis is a very frequent disease in the female gender and is characterized by the presence of endometrial structures outside the uterine cavity. The lack of a reliable non-invasive diagnostic test together with the fact that the symptoms of this pathology are often very non-specific, are responsible for the delay in the definitive diagnosis of this disease. Recently, through a proteomics approach, our research group has identified two potential diagnostic markers for endometriosis in serum (Zn-alpha2-glycoprotein and complement C3 precursor). In this article, we have defined the experimental conditions of a simple ELISA assay for rapid quantification of these two biomarkers in the saliva of patients with endometriosis. Finally, preliminary experiments on a small cohort of patients and controls have confirmed the potential diagnostic value of this assay.
期刊介绍:
Critical ReviewsTM in Eukaryotic Gene Expression presents timely concepts and experimental approaches that are contributing to rapid advances in our mechanistic understanding of gene regulation, organization, and structure within the contexts of biological control and the diagnosis/treatment of disease. The journal provides in-depth critical reviews, on well-defined topics of immediate interest, written by recognized specialists in the field. Extensive literature citations provide a comprehensive information resource.
Reviews are developed from an historical perspective and suggest directions that can be anticipated. Strengths as well as limitations of methodologies and experimental strategies are considered.