用于子宫内膜异位症体外诊断的两种生物标记物定量的唾液测定原型

IF 1.5 4区 医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Pietro G Signorile, Sabrina Dominici, Rosa Viceconte, Alfonso Baldi
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引用次数: 0

摘要

子宫内膜异位症是一种女性常见病,其特点是子宫内膜结构出现在子宫腔以外。由于缺乏可靠的非侵入性诊断检测方法,加之这种病症的症状往往没有特异性,因此这种疾病迟迟得不到明确诊断。最近,通过蛋白质组学方法,我们的研究小组在血清中发现了两种潜在的子宫内膜异位症诊断标志物(Zn-α2-糖蛋白和补体 C3 前体)。在这篇文章中,我们定义了一种简单的 ELISA 检测方法的实验条件,以快速定量检测子宫内膜异位症患者唾液中的这两种生物标记物。最后,在一小批患者和对照组中进行的初步实验证实了这种检测方法的潜在诊断价值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Prototype of a salivary assay for quantification of two biomarkers for in vitro diagnosis of endometriosis
Endometriosis is a very frequent disease in the female gender and is characterized by the presence of endometrial structures outside the uterine cavity. The lack of a reliable non-invasive diagnostic test together with the fact that the symptoms of this pathology are often very non-specific, are responsible for the delay in the definitive diagnosis of this disease. Recently, through a proteomics approach, our research group has identified two potential diagnostic markers for endometriosis in serum (Zn-alpha2-glycoprotein and complement C3 precursor). In this article, we have defined the experimental conditions of a simple ELISA assay for rapid quantification of these two biomarkers in the saliva of patients with endometriosis. Finally, preliminary experiments on a small cohort of patients and controls have confirmed the potential diagnostic value of this assay.
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来源期刊
Critical Reviews in Eukaryotic Gene Expression
Critical Reviews in Eukaryotic Gene Expression 生物-生物工程与应用微生物
CiteScore
2.70
自引率
0.00%
发文量
67
审稿时长
1 months
期刊介绍: Critical ReviewsTM in Eukaryotic Gene Expression presents timely concepts and experimental approaches that are contributing to rapid advances in our mechanistic understanding of gene regulation, organization, and structure within the contexts of biological control and the diagnosis/treatment of disease. The journal provides in-depth critical reviews, on well-defined topics of immediate interest, written by recognized specialists in the field. Extensive literature citations provide a comprehensive information resource. Reviews are developed from an historical perspective and suggest directions that can be anticipated. Strengths as well as limitations of methodologies and experimental strategies are considered.
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