斯里兰卡种植的 Passifloara foetida 的抗氧化、抗糖尿病和抗炎活性

P. G. N. H. Dharmasiri, P. Ranasinghe, P. T. Jayasooriya, K. Samarakoon
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摘要

西番莲(Padagedi)被认为是斯里兰卡一种未充分利用的水果,在传统医学中被用于治疗多种疾病。本研究旨在确定西番莲叶和果的抗氧化、抗糖尿病和抗炎特性。使用甲醇(100%)制备植物提取物。使用总酚含量(TPC)、总黄酮含量(TFC)、总生物碱含量(TAC)、2,2-二苯基-2-苦基肼(DPPH)自由基清除、2,2′-偶氮-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)自由基清除、铁还原抗氧化力(FRAP)和氧自由基吸收能力(ORAC)测定抗氧化活性。抗糖化和抗淀粉酶抑制实验测定了植物提取物的抗糖尿病作用。细胞活力通过 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基-2H-溴化四氮唑(MTT)检测法进行分析。根据研究结果,P. foetida 的叶提取物显示出较高的 TPC(21.46±1.56 mg GAE/g(提取物))、TFC(4.25±0.23 mg QE/g(提取物))、TAC(4.37±6.50 mg/g(提取物))、DPPH(10.91±1.29毫克毒性当量/克提取物)、ABTS+(105.80±18.62毫克毒性当量/克提取物)、FRAP(11.85±1.45毫克毒性当量/克提取物)和 ORAC(46.23±3.58毫克毒性当量/克提取物)。叶提取物的抗淀粉酶活性和抗糖化活性较高(分别为 30.7% 和 77.65%)。与果实提取物相比,P. foetida 的果实提取物对 RAW 巨噬细胞(264.7 细胞)的细胞活力和一氧化氮(NO)产生的抑制率最高。由此可以得出结论,佛手瓜具有良好的治疗特性,可以成为制药和营养保健品行业的潜在来源。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antioxidant, Anti-Diabetic and Anti-Inflammatory Activities of Passifloara foetida grown in Sri Lanka
Passiflora foetida (Padagedi) is identified as an underutilized fruit in Sri Lanka, used in traditional medicine for several diseases. The objective of this study was to determine the antioxidant, anti-diabetic, and anti-inflammatory properties of the leaves and fruits of P. foetida. Methanol (100 %) was used to prepare the plant extracts. The antioxidant activity was determined using total phenolic content (TPC), total flavonoid content (TFC), total alkaloid content (TAC), 2,2-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical scavenging, ferric reducing antioxidant power (FRAP), and oxygen radical absorption capacity (ORAC) assays. The anti-diabetic effects of plant extracts were determined by using anti-glycation and anti-amylase inhibitory assays. Cell viability was analyzed by the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-2H-tetrazolium bromide (MTT) assay. Based on the findings, the leaf extract of P. foetida showed a higher TPC (21.46±1.56 mg GAE/g of extract), TFC (4.25±0.23 mg QE/g of extract), TAC (4.37±6.50 mg/g of extract), DPPH (10.91±1.29 mg TE/g of extract), ABTS+ (105.80±18.62 mg TE/g of extract), FRAP (11.85±1.45 mg TE/g of extract), and ORAC (46.23±3.58 mg TE/g of extract) compared to its fruit extract. The anti-amylase and anti-glycation activities were high in the leaf extract of P. foetida (30.7% and 77.65 % respectively). The fruit extract of P. foetida had the highest cell viability and nitric oxide (NO) production-inhibition in RAW macrophage (264.7 cells) compared to the fruit extract. It can be concluded that P. foetida has good therapeutic properties, and could be a potential source for pharmaceuticals and nutraceuticals industries.
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