Evaluation Of Antimicrobial Effects Of Orthodontic Band Cement Incorporated With Zirconia, Gold, Copper Biosynthesized Nanoparticles- An In-Vitro Study

OBJECTIVE: To evaluate the antimicrobial effects of orthodontic band cement incorporated with various biosynthesized nanoparticles. MATERIALS AND METHODS: Zirconia, gold, copper Nanoparticles were green synthesized using white tea extract and dry ginger which are incorporated into orthodontic band cement, characterized using FTIR analysis. Antimicrobial activity against Streptococcus mutans and Lactobacillus acidophilus was tested in vitro by disc diffusion and Time Kill Curve Assay. RESULTS: Zirconia nanoparticles incorporated GIC exhibited better antimicrobial activity against streptococcus mutans, and gold nanoparticles incorporated GIC against lactobacillus acidophilus at varying concentrations at different time period. CONCLUSION: The antibacterial effect against streptococcus mutans and lactobacillus acidophilus were distinctly noted in Zirconia nanoparticles, gold nanoparticles followed by copper nanoparticles. Zirconia and gold nanoparticles produced more efficient antibacterial property and eventually would be effective in curtailing White Spot Lesions.