伊朗西北部乌尔米耶市宠物猫中五联巴顿氏菌的基因组检测和系统发育分析

IF 2 3区农林科学 Q4 IMMUNOLOGY

Comparative Immunology Microbiology and Infectious Diseases Pub Date : 2024-01-06 DOI:10.1016/j.cimid.2024.102125

Sima Alempour Rajabi , Abdolghaffar Ownagh , Mojtaba Hadian

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引用次数: 0

摘要

本研究的目的是调查伊朗西北部乌尔米耶市宠物猫体内巴顿氏菌五联属的存在情况和遗传特征。研究人员采集了 200 只猫的血液样本，并记录了它们的年龄、性别和品种。采用巢式 PCR 和测序方法鉴定阳性样本中的昆特拉布氏菌，并使用 BioEdit 软件分析 ftsZ 基因序列。本研究获得的基因序列与 GenBank® 数据库中的参考序列相似度为 100.00%，并使用 MEGA11 构建了系统发生树。结果显示，15%的猫（200 份血样中的 30 份）对 B. quintana 基因检测呈阳性，95% 的置信区间为 10.71% 至 20.61%。

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Genomic detection and phylogenetic analysis of Bartonella quintana in pet cats from Urmia City, Northwest Iran

The aim of this study was to investigate the presence and genetic characteristics of Bartonella quintana in pet cats from Urmia City, located in the northwest of Iran. Blood samples were collected from 200 cats, and their age, gender, and breed were noted. Nested-PCR and sequencing were used to identify B. quintana in positive samples, and the ftsZ gene sequences were analyzed using BioEdit software. The gene sequence obtained in this study exhibited 100.00 % similarity to reference sequences in the GenBank® database, and a phylogenetic tree was constructed using MEGA11. The results revealed that 15 % of the cats (30 out of 200 blood samples) tested positive for the B. quintana gene, with a 95 % confidence interval of 10.71 % to 20.61 %.

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来源期刊

Comparative Immunology Microbiology and Infectious Diseases 医学-免疫学

CiteScore

4.60

自引率

0.00%

发文量

102

审稿时长

40 days

期刊介绍： Comparative Immunology, Microbiology & Infectious Diseases aims to respond to the concept of "One Medicine" and to provide a venue for scientific exchange. Based on the concept of "Comparative Medicine" interdisciplinary cooperation between specialists in human and animal medicine is of mutual interest and benefit. Therefore, there is need to combine the respective interest of physicians, veterinarians and other health professionals for comparative studies relevant to either human or animal medicine . The journal is open to subjects of common interest related to the immunology, immunopathology, microbiology, parasitology and epidemiology of human and animal infectious diseases, especially zoonotic infections, and animal models of human infectious diseases. The role of environmental factors in disease emergence is emphasized. CIMID is mainly focusing on applied veterinary and human medicine rather than on fundamental experimental research.