黑木耳甲醇提取物对念珠菌属的抑制试验

Afina Indrawati, Amelya Dyah Pertiwi, Audry Ridho Ayuningtyias, Hillda Widianingtyas Subroto, Mufidhatul Nur Azizah, Tutik Handayani, Surahmaida Surahmaida, Kinanti Ayu Puji Lestari, Floreta Fiska Yuliarni
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引用次数: 0

摘要

念珠菌是一种真菌,可导致念珠菌感染。念珠菌病可能发生在皮肤、指甲、粘膜和胃肠道。本研究旨在探讨黑木耳甲醇提取物是否具有抑制白色念珠菌、光滑念珠菌和副丝状念珠菌生长的能力。提取方法为索氏提取法,抑制试验方法为扩散纸片法和孔扩散法。使用的浓度变化为 0.2 克/毫升、0.25 克/毫升和 0.3 克/毫升,以 10%二甲基亚砜为阴性对照。研究结果表明,在 0.2 克/毫升、0.25 克/毫升和 0.3 克/毫升的浓度下没有形成抑制区,因此它们被列入非活性类别。关键词黑木耳;念珠菌;索氏法;甲醇提取物;纸片法和井氏法。 摘要 念珠菌是一种可引起念珠菌感染的酵母菌。念珠菌病可发生在皮肤、指甲、粘膜和胃肠道。本研究旨在确定黑木耳甲醇提取物抑制白念珠菌、草绿色念珠菌和副丝状念珠菌生长的能力。提取方法为沉降 10 小时,抗真菌抑制试验方法为盘式扩散和点样。使用的浓度变化为 0.2 克/毫升、0.25 克/毫升和 0.3 克/毫升,以 10%二甲基亚砜为阴性对照。结果表明,在 0.2 克/毫升、0.25 克/毫升和 0.3 克/毫升的浓度下均未形成抑制区,因此被列入非活性类别。关键词黑木耳,念珠菌,肉汁法,甲醇提取物,圆盘和井扩散法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Uji Daya Hambat Ekstrak Metanol Auricularia nigricans terhadap Candida spp.
Candida was a fungus that can cause candidiasis infection. Candidiasis could occur of the skin, nails, mucous membranes and the gastrointestinal. The study aims to examine whether Auricularia nigricans methanol extract has the ability to inhibit the growth of Candida albicans, C. glabrata, and C. parapsilosis. Soxhletation was used as the extraction method, and the inhibitory test method involved diffusion paper disk and wells methods. The concentration variations used were 0.2 g/ml, 0.25 g/ml and 0.3 g/ml with 10% DMSO as a negative control. The research results obtained were that no inhibition zones were formed at concentrations of 0.2 g/ml, 0.25 g/ml and 0.3 g/ml so that they were included in the inactive category. Keywords: Auricularia nigricans, Candida, soxhlet method, methanol extract, paper disc and wells method.   Abstrak Candida merupakan jamur golongan khamir yang dapat menyebabkan infeksi kandidiasis. Kandidiasis tersebut dapat terjadi pada kulit, kuku, membran mukosa dan saluran cerna. Penelitian ini bertujuan untuk mengetahui kemampuan ekstrak metanol Auricularia nigricans dalam menghambat pertumbuhan C. albicans, C. glabrata dan C. parapsilosis. Metode ekstraksi yang digunakan adalah sokletasi selama 10 jam dan metode uji daya hambat antifunginya secara difusi cakram dan sumuran. Variasi konsentrasi yang digunakan adalah 0,2 g/ml, 0,25 g/ml dan 0,3 g/ml dengan DMSO 10% sebagai kontrol negatif. Hasil penelitian yang diperoleh adalah tidak terbentuknya zona hambat pada konsentrasi 0,2 g/ml, 0,25 g/ml dan 0,3 g/ml sehingga termasuk dalam kategori tidak aktif. Kata kunci: Auricularia nigricans, Candida, metode sokletasi, ekstrak metanol, metode difusi cakram dan sumuran.
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