{"title":"单链多聚烯酸聚酯（POLY(rA) 和 POLY(rU) 的杂化研究","authors":"Zvart H. Movsisyan","doi":"10.46991/pysu:b/2023.57.2.126","DOIUrl":null,"url":null,"abstract":"The study of the interaction of intercalators ethidium bromide (EtBr), methylene blue (MB) and groove binding compound Hoechst 33258 (H33258) with single-stranded synthetic polyribonucleotides poly(rA) and poly(rU) has been carried out by the method of UV-melting, at ionic strengths of the solution 0.04 M and 0.1 M. Stirring of poly(rA) and poly(rU) by equal-molar concentrations was shown to result in hybridization with formation of double-stranded (ds-) structure poly(rA)-poly(rU). It was revealed that EtBr, with higher degree in comparison with MB, stimulates hybridization and stabilizes the formed ds-structure poly(rA)-poly(rU). It was also found out that the hybridization process and affinity of EtBr and MB depend on the ionic strength of the solution and these processes occur much more effectively at the ionic strength 0.04 M. On the other hand, it was shown that the groove binding ligand H33258 practically does not affect the stabilization of the formed ds-structure poly(rA)-poly(rU).","PeriodicalId":20692,"journal":{"name":"Proceedings of the YSU B: Chemical and Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"STUDY OF HYBRIDIZATION OF COMPLEMENTARY SINGLE-STRANDED POLYNUCLEOTIDES POLY(rA) AND POLY(rU)\",\"authors\":\"Zvart H. Movsisyan\",\"doi\":\"10.46991/pysu:b/2023.57.2.126\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The study of the interaction of intercalators ethidium bromide (EtBr), methylene blue (MB) and groove binding compound Hoechst 33258 (H33258) with single-stranded synthetic polyribonucleotides poly(rA) and poly(rU) has been carried out by the method of UV-melting, at ionic strengths of the solution 0.04 M and 0.1 M. Stirring of poly(rA) and poly(rU) by equal-molar concentrations was shown to result in hybridization with formation of double-stranded (ds-) structure poly(rA)-poly(rU). It was revealed that EtBr, with higher degree in comparison with MB, stimulates hybridization and stabilizes the formed ds-structure poly(rA)-poly(rU). It was also found out that the hybridization process and affinity of EtBr and MB depend on the ionic strength of the solution and these processes occur much more effectively at the ionic strength 0.04 M. On the other hand, it was shown that the groove binding ligand H33258 practically does not affect the stabilization of the formed ds-structure poly(rA)-poly(rU).\",\"PeriodicalId\":20692,\"journal\":{\"name\":\"Proceedings of the YSU B: Chemical and Biological Sciences\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-07-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Proceedings of the YSU B: Chemical and Biological Sciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.46991/pysu:b/2023.57.2.126\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Proceedings of the YSU B: Chemical and Biological Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.46991/pysu:b/2023.57.2.126","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
通过紫外熔融法研究了插入剂溴化乙锭（EtBr）、亚甲基蓝（MB）和沟槽结合化合物 Hoechst 33258（H33258）与单链合成多核苷酸聚（rA）和聚（rU）的相互作用，溶液离子强度分别为 0.04 M 和 0.1 M。等摩尔浓度的聚(rA)和聚(rU)在搅拌后会发生杂交，形成双链（ds-）结构的聚(rA)-聚(rU)。结果表明，与 MB 相比，EtBr 的浓度更高，它能刺激杂交并稳定所形成的 ds 结构聚(rA)-聚(rU)。研究还发现，EtBr 和 MB 的杂化过程和亲和力取决于溶液的离子强度，在离子强度为 0.04 M 时，杂化过程会更有效。
STUDY OF HYBRIDIZATION OF COMPLEMENTARY SINGLE-STRANDED POLYNUCLEOTIDES POLY(rA) AND POLY(rU)
The study of the interaction of intercalators ethidium bromide (EtBr), methylene blue (MB) and groove binding compound Hoechst 33258 (H33258) with single-stranded synthetic polyribonucleotides poly(rA) and poly(rU) has been carried out by the method of UV-melting, at ionic strengths of the solution 0.04 M and 0.1 M. Stirring of poly(rA) and poly(rU) by equal-molar concentrations was shown to result in hybridization with formation of double-stranded (ds-) structure poly(rA)-poly(rU). It was revealed that EtBr, with higher degree in comparison with MB, stimulates hybridization and stabilizes the formed ds-structure poly(rA)-poly(rU). It was also found out that the hybridization process and affinity of EtBr and MB depend on the ionic strength of the solution and these processes occur much more effectively at the ionic strength 0.04 M. On the other hand, it was shown that the groove binding ligand H33258 practically does not affect the stabilization of the formed ds-structure poly(rA)-poly(rU).
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