评估旱芹(Helianthus tuberosum L.)草本植物干提取物的免疫调节活性

Mikhailova I.V., Filippova Y.V., Smolyagin A.I., Sinegovets A.A., Saybel O.L., Dul V.N., Dargaeva T.D.
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引用次数: 0

摘要

.本研究的目的是从细胞抑制剂甲氨蝶呤诱导的实验性免疫缺陷模型小鼠的免疫系统器官重量(胸腺、脾脏)、细胞数量(胸腺、脾脏、骨髓)、细胞免疫和体液免疫指标等方面,评估耶路撒冷朝鲜蓟草干提取物的免疫调节特性。材料和方法在甲氨蝶呤诱导的免疫抑制条件下,研究耶路撒冷朝鲜蓟草本植物干提取物的免疫调节活性,评估其是否能使小鼠免疫系统器官的质量(胸腺、脾脏)、细胞数量(胸腺、脾脏、骨髓)、细胞免疫指标、体液免疫指标以及腹腔巨噬细胞的吞噬活性恢复到完整小鼠的水平。结果分析耶路撒冷朝鲜蓟草本植物干提取物在体内系统中的影响发现,草本植物 Helianthus tuberosum L. 的干提取物对小鼠的胸腺质量(胸腺细胞)没有影响。对免疫系统器官的质量(胸腺、脾脏)和细胞数量(胸腺、脾脏、骨髓)、迟发型超敏反应的严重程度、抗体产生、完整小鼠腹腔巨噬细胞的吞噬活性均无影响,而对使用细胞抑制剂甲氨蝶呤治疗的动物,这些免疫系统参数可恢复到完整动物的水平,与参考药物 "Immunal "的效果相当。结论在甲氨蝶呤诱导的免疫抑制条件下,耶路撒冷朝鲜蓟草本植物的干提取物可被视为一种有前途的制剂,它能将细胞免疫、体液免疫、腹腔巨噬细胞的吞噬活性和免疫系统的细胞组成等参数恢复到完整动物的水平,其效果与参照药物 "Immunal "的效果相当。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
EVALUATION OF THE IMMUNOMODULATORY ACTIVITY OF THE DRY EXTRACT OF THE HERB OF HELIANTHUS TUBEROSUM L.
. The purpose of the study was to evaluate the immunomodulatory properties of the dry extract of Jerusalem artichoke herb in terms of weight (thymus, spleen), number of cells (thymus, spleen, bone marrow) of the immune system organs, indicators of cellular and humoral immunity in mice on a model of experimental immunodeficiency induced by the cytostatic methotrexate. Materials and methods. The study of the immunomodulatory activity of the dry extract of Jerusalem artichoke herb was evaluated by the ability to restore the mass (thymus, spleen), the number of cells (thymus, spleen, bone marrow) of the immune system organs, indicators of cellular, humoral immunity and phagocytic activity of peritoneal macrophages of outbred mice under conditions of immunosuppression induced by methotrexate, to the level of intact mice, in vivo. Results. Analysis of the effect of the dry extract of Jerusalem artichoke herb in in vivo systems found that the dry extract of the herb Helianthus tuberosum L. had no effect on the mass (thymus, spleen) and the number of cells (thymus, spleen, bone marrow) of the immune system organs, the severity of the delayed-type hypersensitivity reaction, antibody production, phagocytic activity of peritoneal macrophages of intact mice and restored these parameters of the immune system in animals treated with the cytostatic methotrexate to the level of intact animals, which was comparable to the effect of the reference drug "Immunal". Conclusion. The dry extract of Jerusalem artichoke herb can be considered as a promising agent that restores the parameters of cellular, humoral immunity, the phagocytic activity of peritoneal macrophages and the cellular composition of the immune system under conditions of immunosuppression induced by methotrexate, to the level of intact animals, the effectiveness of which is comparable to the effect of the comparator drug "Immunal
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