穿心莲植物提取物的植物化学筛选和对特定微生物的抗菌活性

Salma Fathima, Pruthvi Charan Jambiga, Ramya Thumma, Samreen Ahmadi, Srinitha Askani, Bushra Sumayya Mohammed, Poornesh Sutramay, S. Dharavath, S. Taduri
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摘要

背景:穿心莲是一种药用植物,在各种草药系统中的传统使用历史悠久。研究目的本研究旨在对穿心莲的叶、茎和根部分进行植物化学筛选和抗菌活性研究。方法:使用氯仿、丁醇、甲醇、乙醇和水溶剂,通过冷浸渍技术进行初步筛选。植物化学筛选结果显示存在生物碱、苷类、黄酮类、萜类、酚类、单宁和皂苷。采用井扩散法对A. paniculata的叶、茎和根的丁醇提取物进行了抗菌活性检测。研究结果叶、茎和根的外植体提取物中含有大量糖苷。生物碱和黄酮类化合物在叶、茎和根的提取物中含量适中,鞣质、酚类和皂苷含量较少。叶丁醇提取物对大肠杆菌的抑菌面积最大,茎丁醇提取物对枯草杆菌的抑菌面积最大,根丁醇提取物对芽孢杆菌的抑菌面积最大。结论这项研究揭示了 A.Paniculata 中潜在的光化学成分和抗菌活性。这些生物活性物质可用于治疗。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Phytochemical screening and antimicrobial activity of the plant extracts Andrographis paniculata against selected microbes
Background: Andrographis paniculata is a medicinal plant with a long history of traditional use in various herbal medicine systems. Objective: This study aims to conduct aphytochemical screening and antibacterial activity from leaf, stem and root parts of Andrographis paniculata. Methods: The preliminary screening was done by cold maceration technique using chloroform, butanol, methanol, ethanol and aqueous solvents. This phytochemical screening showed the presence of alkaloids, glycosides, flavonoids, terpenoids, phenols, tannins and saponins. Antibacterial activity was performed by using well diffusion method from leaf, stem and root butanolic extracts of A. paniculata. Results: Glycosides are abundantly present in leaf, stem and root explant extracts. Whereas, alkaloids and flavonoids are moderately present in leaf, stem and root explant extracts and followed by less presence of tannins, phenols and saponins. The maximum zone of bacterial inhibition was observed in leaf butanolic extracts against of the E.Coli, for stem butanolic extract against of B. subtilis and for root butanolic extract against B. sphaericus. Conclusion: This study revealed the potential photochemical constituents and antibacterial activity in A.Paniculata. The presence of these bioactive substances they use in treatment.
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