通用 PCR 引物,用于识别蔷薇科三七属及其他成员的物种

Anastasia M. Koltunova, M. G. Kutsev, Ramazan A. Murtazaliev
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引用次数: 0

摘要

我们设计了七种引物,用于通过聚合酶链式反应(PCR)扩增叶绿体 DNA 的非编码区。为了弄清这些引物是否具有通用性,我们使用它们尝试扩增蔷薇科不同植物物种的 DNA 片段。引物适用于番荔枝属的物种和蔷薇科的一些物种。这些引物能在广泛的分类范围内扩增叶绿体 DNA 非编码区,这意味着这些引物可用于研究植物的种群生物学和进化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Universal PCR primers for identifying species of the genus Sanguisorba and other members of the family Rosaceae
Seven primers for the amplification of non-coding regions of chloroplast DNA via the polymerase chain reaction (PCR) have been designed. In order to find out whether these primers were universal, we used them in an attempt to amplify DNA fragments from various plant species of Rosaceae. Primers worked for species of the genus Sanguisorba and some species of the family Rosaceae. The fact that they amplify chloroplast DNA non-coding regions over a wide taxonomic range means that these primers may be used to study the population biology and evolution of plants.
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