Hydroxyalloxanthine Pigment from Garcinia indica

Garcinia indica is the fruit which is reddish purple in colour and is commonly known as kokum or amsul. G. indica is known to have major bioactive compounds viz. Garcinol, Hydroxycitric acid, Kokum butter. One of the major pigments it contains is anthocyanin. The present study was carried out to the isolation of pigment from dried kokum fruit using liquid- liquid extraction. The isolated pigment was identified using HR – LCMS. Further the isolated pigment was analyzed for its antioxidant (DPPH assay), antimicrobial (agar cup method) and cloth dying properties. The isolated pigment was identified as 4-Keto-4'-hydroxyalloxanthine. The pigment showed antimicrobial (24.5 mm zone of clearance) and antioxidant (230μg /ml) activity comparable to the standards ampicillin and ascorbic acid respectively. The extracted pigments gave yellow colour staining on the cotton cloth. The study demonstrated the isolation of 4-Keto-4'-hydroxyalloxanthine from G. indica and its medicinal and cloth dying properties.