抗真菌菌株 M1-8 和 M6-4 作为生物控制剂防治花生仁上的黄曲霉病菌

Yanjie Yi, Youtian Shan, Yu Lou, Zhiwen Ning, Qingyao Zhang, Yingao Yang, Yuqian Liang, Jinsheng Shi, Zhipeng Hou
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引用次数: 0

摘要

黄曲霉菌是花生仁的主要致病真菌,对经济和健康有很大的负面影响。为了探索黄曲霉菌的生物防治菌株,研究人员从霉变物中分离的 70 株细菌中筛选出两株拮抗菌株,并将其鉴定为植物伯克霍尔德氏菌 M1-8 和 Glumae 伯克霍尔德氏菌 M6-4。体外病原体抑制测定表明,M1-8 和 M6-4 的培养滤液具有明显的抑制区,可减少黄曲霉菌的菌丝生长。此外,生物防治试验表明,用培养滤液处理的花生种子的发霉率远远低于对照组。M1-8 和 M6-4 培养滤液的生物防治效果分别达到 88.6% 和 84.2%,高于山梨酸处理组。此外,M1-8 的培养滤液对蛋白酶 K、强光、pH 值和温度(低于 70°C)都有很好的耐受性,抗真菌活性的稳定性也很好。结果表明,这两种抗真菌菌株可用作生物控制剂,在花生供应和储存期间控制黄曲霉。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antifungal strains M1-8 and M6-4 as biocontrol agents against Aspergillus flavus on peanut kernels
Aspergillus flavus is the main pathogenic fungi for peanut kernels, and it has highly negative economic and health impacts. To explore the biological control agent against A. flavus, two antagonistic strains were screened from 70 bacteria isolates from moldy stuff and identified as Burkholderia plantarii M1-8 and Burkholderia glumae M6-4. In vitro pathogen inhibition determination indicated culture filtrates of M1-8 and M6-4 had distinct inhibition zones and could decrease the mycelial growth of A. flavus. Furthermore, the biocontrol assay showed moldy rates of peanut seeds treated with culture filtrate were much lower than that of the control group. The biocontrol efficacy of M1-8 and M6-4 culture filtrate reached 88.6% and 84.2%, respectively, which were higher than that of the sorbic acid treatment group. Moreover, culture filtrate of M1-8 was tolerant to proteinase K, high light, pH and temperature (below 70°C), and had good stability of antifungal activity. The results indicate that these two antifungal strains could be used as biocontrol agents for controlling A. flavus during the peanut supply and storage period.
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