{"title":"免疫毒素 scFv-Mmut 的表达和抗乳腺癌效果","authors":"Shangkun Han","doi":"10.54097/ijbls.v4i1.19","DOIUrl":null,"url":null,"abstract":"In this experiment, the Pichia Pastoris expression plasmid pPIC9K/scFv-Mmut was constructed, and the immunotoxin scFv-Mmut was successfully expressed in the eukaryotic expression system of Pichia Pastoris. The effect and mechanism of immunotoxin scFv-Mmut on inhibiting activity and inducing apoptosis of breast cancer cells were analyzed by in vitro assay. Through this experiment, it can be found that after the treatment of BT474 cells with a certain concentration of scFv-Mmut, the cell cycle will be affected and be blocked in the S phase, which proves that scFv-Mmut can induce the apoptosis of BT474 cells and has a significant anti-breast cancer effect.","PeriodicalId":507854,"journal":{"name":"International Journal of Biology and Life Sciences","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Expression and Anti-breast Cancer Effect of Immunotoxin scFv-Mmut\",\"authors\":\"Shangkun Han\",\"doi\":\"10.54097/ijbls.v4i1.19\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"In this experiment, the Pichia Pastoris expression plasmid pPIC9K/scFv-Mmut was constructed, and the immunotoxin scFv-Mmut was successfully expressed in the eukaryotic expression system of Pichia Pastoris. The effect and mechanism of immunotoxin scFv-Mmut on inhibiting activity and inducing apoptosis of breast cancer cells were analyzed by in vitro assay. Through this experiment, it can be found that after the treatment of BT474 cells with a certain concentration of scFv-Mmut, the cell cycle will be affected and be blocked in the S phase, which proves that scFv-Mmut can induce the apoptosis of BT474 cells and has a significant anti-breast cancer effect.\",\"PeriodicalId\":507854,\"journal\":{\"name\":\"International Journal of Biology and Life Sciences\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-11-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Biology and Life Sciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.54097/ijbls.v4i1.19\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Biology and Life Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.54097/ijbls.v4i1.19","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Expression and Anti-breast Cancer Effect of Immunotoxin scFv-Mmut
In this experiment, the Pichia Pastoris expression plasmid pPIC9K/scFv-Mmut was constructed, and the immunotoxin scFv-Mmut was successfully expressed in the eukaryotic expression system of Pichia Pastoris. The effect and mechanism of immunotoxin scFv-Mmut on inhibiting activity and inducing apoptosis of breast cancer cells were analyzed by in vitro assay. Through this experiment, it can be found that after the treatment of BT474 cells with a certain concentration of scFv-Mmut, the cell cycle will be affected and be blocked in the S phase, which proves that scFv-Mmut can induce the apoptosis of BT474 cells and has a significant anti-breast cancer effect.
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