水稻基因型对细菌性枯萎病的抗性筛选

Jyoti P. Jirankali, C. A. Deepak, B. Chethana, G. M. Sandesh, G. Nikhil, M. Rajanna
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摘要

2020 年期间,在印度卡纳塔克邦曼迪亚的 V. C. 农场开展了一项研究,以筛选在自然田间条件下抗蓝枯病的基因型。寄主植物抗性是该病害综合防治计划的重要组成部分。在曼迪亚 V. C. 农场区级农业站自然附生条件下筛选的 102 个水稻基因型中,没有一个对细菌性叶枯病有免疫力。 因此,在研究中,通过测量病害严重程度(病叶百分比)和病害进展曲线下面积(AUDPC)来评估植株。在 IRRI 水稻栽培品种上测试了 Xoo 的致病性,在最大分蘖期接种,接种 14 天后测量病斑长度。通过剪切法人工接种,尝试对 IRRI 的 102 个基因型进行表型鉴定,以确定其对 BLB 的抗性。在测试的 102 个基因型和两个对照中,观察到五个条目,即IRGC 125853、IRGC 126264、IRGC 132357、IRGC 122088、IRGC 125658 和改良 Samba Mahsuri 为高抗性,得分为 1 分;只有一个品系(IRGC 125754)为抗性,得分为 3 分;33 个品系为中抗性,得分为 5 分;43 个品系为感病,得分为 7 分;20 个品系和感病对照 Jyothi-PTB 39 的表型得分最高,得分为 9 分。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Screening of Rice Genotypes for Resistance Against Bacterial Blight Disease
A study was undertaken at V. C. Farm, Mandya, Karnataka, India during 2020 to screen the genotypes against BLB under natural field conditions. Host Plant resistance is an important component of an integrated management program for this disease. Among the 102 rice genotypes screened under natural epiphytotic condition at Zonal Agricultural Station, V. C. Farm, Mandya, none of them were found immune against bacterial leaf blight.  So, in the study, plants were assessed by measuring disease severity (% of leaf diseased) and area under the disease progress curve (AUDPC). The pathogenicity of Xoo was tested on IRRI rice cultivars, inoculation was conducted at the maximum tillering stage, and the lesion length was measured after 14 days of inoculation. An attempt was made to phenotypically characterize a set of 102 Genotypes from IRRI for BLB resistance by artificially inoculaton using clipping method. Out of the 102 genotypes and two checks tested, it was observed that five entries viz., IRGC 125853, IRGC 126264, IRGC 132357, IRGC 122088 IRGC 125658 and Improved Samba Mahsuri were highly resistant with score of 1. Only one entry (IRGC 125754) was resistant with score of 3, 33 lines being moderately resistant with score of 5 and 43 lines were susceptible with score of 7. 20 lines and also the susceptible check Jyothi-PTB 39, had the highest susceptibility with a phenotypic score of 9.
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