测定薤白、茱丽叶和牡丹的抗氧化、抗菌活性、总酚和类黄酮含量

A. Karasakal, Orhan Kiliç, Nazan Tokatlı Demirok, E. Cabi̇
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引用次数: 0

摘要

采用微波辅助萃取(MAE)工艺从 Allium rumelicum Koçyigit & Özhatay、Jurinea kilaea Azn.和 Peucedanum obtusifolium Sibth. & Sm.中提取多酚。该研究考察了从这三种植物中提取的甲醇提取物的抗氧化、抗菌、总酚和类黄酮含量。通过使用 2,2-二苯基-1-吡啶-肼水自由基法(DPPH)、ABTS/过硫酸盐法和铜还原抗氧化能力法(CUPRAC),检测了总抗氧化活性和能力。此外,还采用了 Folin-Ciocalteu 和 AlCl3/KAc 技术来计算总酚和类黄酮的含量。为了确定植物的抗菌能力,采用了盘扩散法。在使用 CUPRAC 和 ABTS/过硫酸盐技术测量时,茱莉叶的总抗氧化能力/活性水平最高。发现薤白的槲皮素浓度最高,而牡丹皮的浓度最低。茱莉叶的没食子酸浓度最高。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Determination of Antioxidant, Antimicrobial Activities, Total Phenolic and Flavonoid Contents of Allium rumelicum, Jurinea kilaea, Peucedanum obtusifolium
A microwave-assisted extraction (MAE) process for polyphenols from Allium rumelicum Koçyigit & Özhatay, Jurinea kilaea Azn., Peucedanum obtusifolium Sibth. & Sm. was used. This research examined the methanolic extracts made from these three species' antioxidant, antimicrobial, total phenolic, and flavonoid contents. By using the 2,2-diphenyl-1-picryl-hydrazyl-hydrate free radical method (DPPH), ABTS/Persulfate, and Cupric reducing antioxidant capacity (CUPRAC) methods, the total antioxidant activities and capacities were examined. Additionally, the Folin-Ciocalteu and AlCl3/KAc techniques were used to calculate the total phenolic and flavonoid contents. In order to ascertain the antibacterial capabilities of plants, the disc diffusion method was applied. The Jurinea kilaea showed the greatest total antioxidant capacity/activity levels when measured using the CUPRAC and ABTS/Persulfate techniques. The Allium rumelicum was found to have the highest quercetin concentration, while Peucedanum obtusifolium had the lowest. In Jurinea kilaea, the gallic acid concentration was greatest.The highest antimicrobial activity values were obtained in Peucedanum obtusifolium
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