{"title":"新英格兰白尾鹿(Odocoileus virginianus)的线粒体 DNA 序列变异:南塔克特岛创始人效应的证据","authors":"Richard Beckwitt, Sarah Bois, Bryan Connolly","doi":"10.1656/045.030.0401","DOIUrl":null,"url":null,"abstract":"Abstract - Odocoileus virginianus (White-tailed Deer) currently number ∼3000 on Nantucket Island. No deer were seen on the island at the beginning of the 20th century. The historical record suggests that a single male deer was brought to the island in 1922, and that 2 female deer were brought to the island from Michigan in 1926. After the deer population had increased to several hundred, additional deer (2 male and 3 female) were brought to Nantucket from New Hampshire in 1935 and 1936. To investigate the presence of founder effect in the population on Nantucket, we obtained samples of White-tailed Deer feces or muscle tissue from Nantucket; the New England mainland (including Cape Cod, southeastern Massachusetts, and a few samples from Maine, Connecticut, and Rhode Island), Shelter Island, NY; and Ann Arbor, MI. We amplified a portion of the mitochondrial control region (D-loop), and found 3 different sequences (haplotypes) among 35 deer samples from Nantucket. Two common haplotypes were identical or nearly identical to haplotypes from Michigan. One rare haplotype was also found in deer from the mainland in Connecticut and Massachusetts. This latter haplotype was unusual in that it contained 3 tandem copies of a 75 base-pair repeat, while most White-tailed Deer have 2 copies. In contrast, we found 5 haplotypes among 26 deer from the New England mainland. Haplotype diversity on Nantucket was 0.447 (± 0.082), and nucleotide diversity (π) was 0.021 (± 0.005). Haplotype diversity on the mainland was 0.839 (± 0.029), and π was 0.046 (± 0.002). Analysis of molecular variance (AMOVA) indicated little genetic differentiation among populations on the New England mainland (ϕST = 0.095, P = 0.113). However, when the population on Nantucket was included in the analysis, there was much more genetic variation among populations (ϕST = 0.414, P = 0.000). Our results indicate that most deer on Nantucket originated from 2 founding females from Michigan, and a small percentage are descended from later introductions from the New England mainland.","PeriodicalId":49742,"journal":{"name":"Northeastern Naturalist","volume":"103 1","pages":"382 - 392"},"PeriodicalIF":0.5000,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Mitochondrial DNA Sequence Variation in White-Tailed Deer (Odocoileus virginianus) in New England: Evidence for Founder Effect on Nantucket Island\",\"authors\":\"Richard Beckwitt, Sarah Bois, Bryan Connolly\",\"doi\":\"10.1656/045.030.0401\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Abstract - Odocoileus virginianus (White-tailed Deer) currently number ∼3000 on Nantucket Island. No deer were seen on the island at the beginning of the 20th century. The historical record suggests that a single male deer was brought to the island in 1922, and that 2 female deer were brought to the island from Michigan in 1926. After the deer population had increased to several hundred, additional deer (2 male and 3 female) were brought to Nantucket from New Hampshire in 1935 and 1936. To investigate the presence of founder effect in the population on Nantucket, we obtained samples of White-tailed Deer feces or muscle tissue from Nantucket; the New England mainland (including Cape Cod, southeastern Massachusetts, and a few samples from Maine, Connecticut, and Rhode Island), Shelter Island, NY; and Ann Arbor, MI. We amplified a portion of the mitochondrial control region (D-loop), and found 3 different sequences (haplotypes) among 35 deer samples from Nantucket. Two common haplotypes were identical or nearly identical to haplotypes from Michigan. One rare haplotype was also found in deer from the mainland in Connecticut and Massachusetts. This latter haplotype was unusual in that it contained 3 tandem copies of a 75 base-pair repeat, while most White-tailed Deer have 2 copies. In contrast, we found 5 haplotypes among 26 deer from the New England mainland. Haplotype diversity on Nantucket was 0.447 (± 0.082), and nucleotide diversity (π) was 0.021 (± 0.005). Haplotype diversity on the mainland was 0.839 (± 0.029), and π was 0.046 (± 0.002). Analysis of molecular variance (AMOVA) indicated little genetic differentiation among populations on the New England mainland (ϕST = 0.095, P = 0.113). However, when the population on Nantucket was included in the analysis, there was much more genetic variation among populations (ϕST = 0.414, P = 0.000). Our results indicate that most deer on Nantucket originated from 2 founding females from Michigan, and a small percentage are descended from later introductions from the New England mainland.\",\"PeriodicalId\":49742,\"journal\":{\"name\":\"Northeastern Naturalist\",\"volume\":\"103 1\",\"pages\":\"382 - 392\"},\"PeriodicalIF\":0.5000,\"publicationDate\":\"2023-11-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Northeastern Naturalist\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://doi.org/10.1656/045.030.0401\",\"RegionNum\":4,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIODIVERSITY CONSERVATION\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Northeastern Naturalist","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1656/045.030.0401","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIODIVERSITY CONSERVATION","Score":null,"Total":0}
Mitochondrial DNA Sequence Variation in White-Tailed Deer (Odocoileus virginianus) in New England: Evidence for Founder Effect on Nantucket Island
Abstract - Odocoileus virginianus (White-tailed Deer) currently number ∼3000 on Nantucket Island. No deer were seen on the island at the beginning of the 20th century. The historical record suggests that a single male deer was brought to the island in 1922, and that 2 female deer were brought to the island from Michigan in 1926. After the deer population had increased to several hundred, additional deer (2 male and 3 female) were brought to Nantucket from New Hampshire in 1935 and 1936. To investigate the presence of founder effect in the population on Nantucket, we obtained samples of White-tailed Deer feces or muscle tissue from Nantucket; the New England mainland (including Cape Cod, southeastern Massachusetts, and a few samples from Maine, Connecticut, and Rhode Island), Shelter Island, NY; and Ann Arbor, MI. We amplified a portion of the mitochondrial control region (D-loop), and found 3 different sequences (haplotypes) among 35 deer samples from Nantucket. Two common haplotypes were identical or nearly identical to haplotypes from Michigan. One rare haplotype was also found in deer from the mainland in Connecticut and Massachusetts. This latter haplotype was unusual in that it contained 3 tandem copies of a 75 base-pair repeat, while most White-tailed Deer have 2 copies. In contrast, we found 5 haplotypes among 26 deer from the New England mainland. Haplotype diversity on Nantucket was 0.447 (± 0.082), and nucleotide diversity (π) was 0.021 (± 0.005). Haplotype diversity on the mainland was 0.839 (± 0.029), and π was 0.046 (± 0.002). Analysis of molecular variance (AMOVA) indicated little genetic differentiation among populations on the New England mainland (ϕST = 0.095, P = 0.113). However, when the population on Nantucket was included in the analysis, there was much more genetic variation among populations (ϕST = 0.414, P = 0.000). Our results indicate that most deer on Nantucket originated from 2 founding females from Michigan, and a small percentage are descended from later introductions from the New England mainland.
期刊介绍:
The Northeastern Naturalist covers all aspects of the natural history sciences of terrestrial, freshwater, and marine organisms and the environments of the northeastern portion of North America, roughly bounded from Virginia to Missouri, north to Minnesota and Nunavut, east to Newfoundland, and south back to Virginia. Manuscripts based on field studies outside of this region that provide information on species within this region may be considered at the Editor’s discretion.
The journal welcomes manuscripts based on observations and research focused on the biology of terrestrial, freshwater, and marine organisms and communities as it relates to their life histories and their function within, use of, and adaptation to the environment and the habitats in which they are found, as well as on the ecology and conservation of species and habitats. Such studies may encompass measurements, surveys, and/or experiments in the field, under lab conditions, or utilizing museum and herbarium specimens. Subject areas include, but are not limited to, anatomy, behavior, biogeography, biology, conservation, evolution, ecology, genetics, parasitology, physiology, population biology, and taxonomy. Strict lab, modeling, and simulation studies on natural history aspects of the region, without any field component, will be considered for publication as long as the research has direct and clear significance to field naturalists and the manuscript discusses these implications.