另一种固定组织学标本的方法是使用热板在 60°c 温度下固定 10 分钟

Jurnal Bahana Kesehatan Masyarakat (Bahana of Journal Public Health) Pub Date : 2023-11-30 DOI:10.35910/jbkm.v7i2.683

Burhannudin Burhannudin, Warida Warida, Indah Puspita

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引用次数: 0

摘要

背景：在保证质量的前提下，使用 60°C 的微波加热 2-10 分钟可缩短组织制备过程中的固定时间。然而，热板在这一过程中的使用仍然有限。本研究旨在确定 60°C 热板加热固定的最佳持续时间。方法：采用后测对照组设计进行实验研究。研究包括两个对照组（常规组和微波组）和三个处理组的 20 份组织学标本，热板加热持续时间分别为 3 分钟（W1）、5 分钟（W2）和 10 分钟（W3）。根据染色结果对组织切片进行评估，并使用评分系统（0-3）进行评分。评分采用 Kruskal-Wallis 和 Mann-Whitney 检验，与对照组相比，显著性水平为 p0.05），而 W2 和 W3 组的结果与对照组相比有显著差异（p<0.05）。结论：使用温度为 60°C 的热板 10 分钟可有效加快固定过程，同时不会影响组织学标本的血苏木精和伊红染色质量。

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AN ALTERNATIVE TO FIXATING HISTOLOGY SPECIMENS BY USING HOTPLATE AT 60°C FOR 10 MINUTES

Background: The fixation process in histological preparation has been shortened by using microwave heating at 60°C for 2-10 minutes while maintaining the quality. However, the use of hotplates in this process is still limited. The study aims to determine the optimal duration of hotplate heating at 60°C for fixation. Method: An experimental study was conducted using a post-test-only control group design. The study includes 20 histological specimens from two control groups (conventional and microwave) and three treatment groups with hotplate heating durations of 3 minutes (W1), 5 minutes (W2) and 10 minutes (W3). Histological slides were evaluated based on staining results and scored using a scoring system (0-3). The scoring was tested using the Kruskal-Wallis and Mann-Whitney tests, with a significance level of p<0.05. Result: microscopic evaluation of the specimens revealed dark staining with Haematoxylin and Eosin in the W2 and W3 groups. However, the W1 group showed similar results (p>0.05) compared to the control group, while the W2 and W3 groups indicated significantly different results (p<0.05) compared to the control group. Conclusion: using a hotplate at 60°C for 10 minutes effectively accelerated the fixation process without compromising the quality of Haematoxylin and Eosin staining in histological specimens.

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Jurnal Bahana Kesehatan Masyarakat (Bahana of Journal Public Health)

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