莫斯科国立罗蒙诺索夫大学生物系的冷冻电镜技术

A. V. Moiseenko, A.M. Egorov, K. V. Shaitan, O. S. Sokolova
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引用次数: 0

摘要

本文展示了将一台 JEOL JEM-2100 分析透射电子显微镜成功升级为低分辨率冷冻电子显微镜的实例,该显微镜专为样品制备和质量评估等常规任务而设计。升级后,该仪器可实现亚纳米分辨率的蛋白质分子重构(8 Å 以内)。讨论了石墨烯和无定形碳支撑膜对防止冷冻样品中蛋白质颗粒优先取向的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cryo-electron microscopy at the Faculty of Biology of Lomonosov Moscow State University
This paper demonstrates an example of a successful upgrade of a JEOL JEM-2100 analytical transmission electron microscope to a low-resolution cryo-electron microscope designed for routine tasks of sample preparation and quality evaluation. As a result of the upgrade, the instrument allows the subnanometer resolution of protein molecule reconstructions (within 8 Å). The influence of graphene and amorphous carbon support films to prevent the effect of preferred orientation of protein particles in the frozen sample is discussed.
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