利用微卫星标记对伊拉克本地奶牛的遗传多样性及其与进口奶牛的比较

IF 1.1 Q3 AGRICULTURE, MULTIDISCIPLINARY

IRAQI JOURNAL OF AGRICULTURAL SCIENCES Pub Date : 2023-12-29 DOI:10.36103/ijas.v54i6.1852

S. M. Al-Jubori, Riyadh Hamad Senkal, R. Prof

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引用次数: 0

摘要

本研究使用 100 头不同品种的奶牛，对微卫星标记 CSSM66、ETH10 和 ILSTS34 的遗传多样性进行了鉴定：经 PCR 反应扩增后，在研究的牛样本中检测到 16 个等位基因，分别为 ETH10、CSSM66 和 ILSTS34，它们分别是 7、6 和 5 个等位基因。总香农（I）值为 1.5400，并且发现 Obs_Hom 的平均值小于 Exp_Het 的平均值，分别为 0.2600 和 0.7544。这表明奶牛之间存在较高的遗传异质性或多样性。FIS、FIT 和 FST 以及遗传迁移 Nm 的平均值分别为 -0.6257、-0.0162、0.3749 和 0.4168。这些结果通过 UPGMA 树显示了家牛与婆罗门牛之间的共同祖先。

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GENETIC DIVERSITY OF IRAQI LOCAL COWS AND THEIR COMPARISON WITH IMPORTED COWS USING MICROSATELLITE MARKERS

This study was conducted to identify the genetic diversity of microsatellite markers CSSM66, ETH10 and ILSTS34 using 100 cows of several breeds distributed as follows: 30 local Iraqi cows (equally divided into Restaki, Janoubi and Sharabi) as well as 50 Holstein cows (German) and 20 Brahman bulls after amplification in PCR reaction, 16 alleles of ETH10, CSSM66 and ILSTS34 markers were detected in a sample of studied cattle and they were 7, 6 and 5 alleles, respectively. The total Shannon (I) was 1.5400, and it was found that the average value of Obs_Hom is smaller than the average value of Exp_Het, being 0.2600 and 0.7544, respectively. This indicates the presence of high genetic heterogeneity or diversity among cows. The average values of FIS, FIT and FST and genetic migration Nm -0.6257, -0.0162, 0.3749 and 0.4168, respectively. These results showed through the UPGMA tree a common ancestry between domestic cattle and Brahman.

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来源期刊

IRAQI JOURNAL OF AGRICULTURAL SCIENCES AGRICULTURE, MULTIDISCIPLINARY-

CiteScore

2.30

自引率

50.00%

发文量

140

审稿时长

6 weeks

期刊介绍： IRAQI JOURNAL OF AGRICULTURAL SCIENCES (IJAS)is the first agric. scientific and refereed journal established in Iraq. The first volume was published in 1966. IJAS is registered in the number 137 in 1988 of the Baghdad National Library. Years ago, it was published with one issue a year. For the time being, it is published bimonthly (6 issues for a volume). IJAS Deal with: Field Crops. Plant Breeding. Agricultural Economics. Agricultural Extension. Agricultural Mechanization. Basic sciences. Hort. Sciences. Animal Husbandry. Food Technology, Plant Pathology. Plant Entomology. Poultry Sciences. Soil Sciences. Water Resources. Veterinary. Biology.