Jakub Mlost, Magdalena Białoń, Marta Kędziora, Agnieszka Wąsik, Żaneta Michalec, Katarzyna Starowicz
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Neurochemical assays were conducted at weeks 3, 6, and 10 post-MIA injection, followed by the neurotransmitters and their metabolites correlation matrix and network analysis.</p><p><strong>Results: </strong>OA animals developed rapid pain phenotype, whereas anxiety-like behavior accompanied the development of a pain phenotype from 6 week post-MIA injection. We did not detect any depressive-like behavior. Instead, immobility time measured in the forced swimming test transiently decreased at 3 weeks post-MIA in the OA group. We detected changes in noradrenaline and serotonin levels in analyzed structures at distinct time points. Network analysis revealed noradrenaline and serotonin neurotransmission changes in the nucleus accumbens, confirming it to be the key structure affected by chronic pain.</p><p><strong>Conclusion: </strong>Animals with chronic pain exhibit symptoms of anxiety-like behavior and we identified underlying neurochemical changes using network analysis.</p>","PeriodicalId":19947,"journal":{"name":"Pharmacological Reports","volume":null,"pages":null},"PeriodicalIF":3.6000,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10830664/pdf/","citationCount":"0","resultStr":"{\"title\":\"Network analysis of monoamines involved in anxiety-like behavior in a rat model of osteoarthritis.\",\"authors\":\"Jakub Mlost, Magdalena Białoń, Marta Kędziora, Agnieszka Wąsik, Żaneta Michalec, Katarzyna Starowicz\",\"doi\":\"10.1007/s43440-023-00562-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Chronic pain is a major health problem that affects a significant number of patients, resulting in personal suffering and substantial health care costs. 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引用次数: 0
摘要
背景:慢性疼痛是一个重大的健康问题,影响着大量患者,造成个人痛苦和大量医疗费用。骨关节炎(OA)是最常见的病因之一。除了感觉症状外,慢性疼痛还与情绪或焦虑症有内在的重叠。人们对额叶皮层、纹状体和伏隔核参与疼痛情感处理的情况仍知之甚少:方法:雄性 Wistar 大鼠分为两组:方法:将雄性 Wistar 大鼠分为两组:MIA 组(向膝关节注射单碘乙酸盐-OA 模型)和假组(氯化钠)。在第 1、3、4、6、8 和 10 周进行行为测试,评估疼痛、焦虑和抑郁行为。在注射 MIA 后的第 3、6 和 10 周进行神经化学测定,然后进行神经递质及其代谢物相关矩阵和网络分析:结果:注射 MIA 后 6 周,OA 动物出现了快速疼痛表型,而焦虑样行为则伴随着疼痛表型的发展。我们没有发现任何类似抑郁的行为。相反,在注射 MIA 后 3 周,OA 组动物在强迫游泳测试中的静止时间短暂缩短。我们在不同的时间点检测到分析结构中去甲肾上腺素和血清素水平的变化。网络分析显示,去甲肾上腺素和血清素神经递质在伏隔核中发生了变化,证实伏隔核是受慢性疼痛影响的关键结构:结论:慢性疼痛动物表现出类似焦虑行为的症状,我们通过网络分析确定了其潜在的神经化学变化。
Network analysis of monoamines involved in anxiety-like behavior in a rat model of osteoarthritis.
Background: Chronic pain is a major health problem that affects a significant number of patients, resulting in personal suffering and substantial health care costs. One of the most commonly reported causal conditions is osteoarthritis (OA). In addition to sensory symptoms, chronic pain shares an inherent overlap with mood or anxiety disorders. The involvement of the frontal cortex, striatum and nucleus accumbens, in the affective processing of pain is still poorly understood.
Methods: Male Wistar rats were divided into two groups: MIA (monoiodoacetate injected into the knee-model of OA) and sham (NaCl). Behavioral tests assessing pain, anxiety, and depressive behavior were performed at week 1, 3, 4, 6, 8, and 10. Neurochemical assays were conducted at weeks 3, 6, and 10 post-MIA injection, followed by the neurotransmitters and their metabolites correlation matrix and network analysis.
Results: OA animals developed rapid pain phenotype, whereas anxiety-like behavior accompanied the development of a pain phenotype from 6 week post-MIA injection. We did not detect any depressive-like behavior. Instead, immobility time measured in the forced swimming test transiently decreased at 3 weeks post-MIA in the OA group. We detected changes in noradrenaline and serotonin levels in analyzed structures at distinct time points. Network analysis revealed noradrenaline and serotonin neurotransmission changes in the nucleus accumbens, confirming it to be the key structure affected by chronic pain.
Conclusion: Animals with chronic pain exhibit symptoms of anxiety-like behavior and we identified underlying neurochemical changes using network analysis.
期刊介绍:
Pharmacological Reports publishes articles concerning all aspects of pharmacology, dealing with the action of drugs at a cellular and molecular level, and papers on the relationship between molecular structure and biological activity as well as reports on compounds with well-defined chemical structures.
Pharmacological Reports is an open forum to disseminate recent developments in: pharmacology, behavioural brain research, evidence-based complementary biochemical pharmacology, medicinal chemistry and biochemistry, drug discovery, neuro-psychopharmacology and biological psychiatry, neuroscience and neuropharmacology, cellular and molecular neuroscience, molecular biology, cell biology, toxicology.
Studies of plant extracts are not suitable for Pharmacological Reports.