通过基因表达总库数据库鉴定老年大鼠大脑中动脉闭塞的枢纽基因和通路

IF 0.8 4区医学 Q4 IMMUNOLOGY

Critical Reviews in Immunology Pub Date : 2023-12-01 DOI:10.1615/critrevimmunol.2023051702

jing Guo, Yi-Zhi Yan, jinglou chen, Yang Duan, Peng Zeng

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引用次数: 0

摘要

脑卒中仍然是世界上最主要的致残原因，而最重要的不可改变风险因素是年龄。由于脑卒中的发病机制复杂，老年患者的治疗面临多重困难。因此，我们旨在确定老年中风患者潜在的差异表达基因（DEGs）和信号通路。比较有无大脑中动脉闭塞（MCAO）的老年大鼠的 DEGs，分析参与老年大鼠脑缺血发病的重要基因和关键信号通路。基因表达总库（GEO）分析工具用于分析 GSE166162 数据集中老年 MCAO 大鼠与老年假大鼠相比的 DEGs。使用 limma 对老年 MCAO 大鼠和假大鼠进行了差异表达分析。此外，还利用 STRING 数据库（12.0 版）构建了 74 个 DEGs（如 Fam111a、Lcn2、Spp1、Lgals3 和 Gpnmb 上调；Egr2、Nr4a3、Arc、Klf4 和 Nr4a1 下调）以及与蛋白质-蛋白质相互作用（PPI）网络中前 20 个核心基因相对应的潜在化合物。在这 30 种化合物中，白藜芦醇、大麻二酚、霍诺可醇、褐藻黄质、齐墩果苷和酪醇明显富集。对这些DEGs进行了基因本体（GO）功能分析和京都基因和基因组百科全书（KEGG）通路富集分析，以确定在老年MCAO大鼠中最显著富集的通路。此外，先天性免疫反应、补体和凝血级联信号通路、IL-17和其他信号通路与老年MCAO大鼠显著相关。我们的研究

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Identification of hub genes and pathways of middle cerebral artery occlusion in aged rats via the Gene Expression Omnibus database

Stroke remained the leading cause of disability in the world, and the most important non-modifiable risk factor was age. The treatment of stroke for elder patients faced multiple difficulties due to its complicated pathogenesis and mechanism. Therefore, we aimed to identify the potential differentially expressed genes (DEGs) and singnalling pathways for aged people of stroke. To compare the DEGs in the aged rats with or without middle cerebral artery occlusion (MCAO) and to analyse the important genes and the key signalling pathways involved in the development of cerebral ischaemia in aged rats. The Gene Expression Omnibus (GEO) analysis tool was used to analyse the DEGs in the GSE166162 dataset of aged MCAO rats compared with aged sham rats. Differential expression analysis was performed in aged MCAO rats and sham rats using limma. In addition, the 74 DEGs (such as Fam111a, Lcn2, Spp1, Lgals3 and Gpnmb were up-regulated; Egr2, Nr4a3, Arc, Klf4 and Nr4a1 were down-regulated) and potential compounds corresponding to the top 20 core genes in the Protein-Protein Interaction (PPI) network was constructed using the STRING database (version 12.0). Among these 30 compounds, resveratrol, cannabidiol, honokiol, fucoxanthin, oleandrin and tyrosol were significantly enriched. These DEGs were subjected to Gene Ontology (GO) function analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis to determine the most significantly enriched pathway in aged MCAO rats. Moreover, innate immune response, the complement and coagulation cascades signalling pathway, the IL-17 and other signalling pathways were significantly correlated with the aged MCAO rats. Our stu

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来源期刊

Critical Reviews in Immunology 医学-免疫学

CiteScore

2.60

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Immunology covers a broad spectrum of investigations at the genes, molecular, cellular, organ and system levels to reveal defense mechanisms against pathogens as well as protection against tumors and autoimmune diseases. The great advances in immunology in recent years make this field one of the most dynamic and rapidly growing in medical sciences. Critical ReviewsTM in Immunology (CRI) seeks to present a balanced overview of contemporary adaptive and innate immune responses related to autoimmunity, tumor, microbe, transplantation, neuroimmunology, immune regulation and immunotherapy from basic to translational aspects in health and disease. The articles that appear in CRI are mostly obtained by invitations to active investigators. But the journal will also consider proposals from the scientific community. Interested investigators should send their inquiries to the editor before submitting a manuscript.