叶尔羌三疣梭子蟹 55 个 SNP 标记的分离与鉴定

IF 0.9 4区环境科学与生态学 Q4 BIODIVERSITY CONSERVATION

Conservation Genetics Resources Pub Date : 2023-12-27 DOI:10.1007/s12686-023-01345-z

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引用次数: 0

摘要

摘要叶尔羌鲑（Triplophysa (Hedinichthys) yarkandensis macroptera）是中国特有物种，主要分布于甘肃省。迄今为止，对该物种的研究非常有限，主要集中在其分类学方面，没有任何可用的分子标记。在本研究中，我们鉴定了55个SNP标记。小等位基因频率范围为 0.050 至 0.500，观察杂合度和预期杂合度范围分别为 0.167 至 1.000 和 0.155 至 0.508。多态性信息含量为 0.141 至 0.375。经 Bonferroni 校正后，这些 SNP 标记中有 17 个位点明显偏离 Hardy-Weinberg 平衡。这些新的SNP标记将有助于该物种未来的遗传管理和种群保护研究。

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Isolation and characterization of 55 SNP markers in Triplophysa (Hedinichthys) yarkandensis macroptera

Abstract

Triplophysa (Hedinichthys) yarkandensis macroptera is an endemic species in China, that is mainly distributed in Gansu Province. To date, research on this species has been very limited, focusing mainly on its taxonomy, without any available molecular markers. In the present study, we identified 55 SNP markers in T. (Hedinichthys) yarkandensis macroptera. The minor allele frequency ranged from 0.050 to 0.500, and the observed and expected heterozygosity ranged from 0.167 to 1.000 and 0.155 to 0.508, respectively. The polymorphic information content ranged from 0.141 to 0.375. Among these SNP markers, seventeen loci showed significant departures from Hardy-Weinberg equilibrium after Bonferroni correction. These novel SNP markers will be helpful for future studies on genetic management and population conservation in this species.

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来源期刊

Conservation Genetics Resources BIODIVERSITY CONSERVATION-GENETICS & HEREDITY

CiteScore

2.90

自引率

9.10%

发文量

审稿时长

3-6 weeks

期刊介绍： Conservation Genetics Resources promotes the conservation of genetic diversity and advances the study of conservation genetics by providing rapid publication of technical papers and reviews on methodological innovations or improvements, computer programs, and genomic resources, as well as on the practical application of these resources towards the development of effective conservation policy and practice.