粒细胞分化过程中表面 CD 标记的系统分析

Q3 Biochemistry, Genetics and Molecular Biology
S E Novikova, T V Tolstova, N A Soloveva, T E Farafonova, O V Tikhonova, L K Kurbatov, A L Rusanov, V G Zgoda
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引用次数: 0

摘要

具有细胞外暴露结构域的质膜蛋白负责将细胞外信号转导为细胞内反应,它们对治疗分子的可及性使其成为药物开发的诱人靶标。在这项工作中,我们利用全量组学技术和免疫化学方法,研究了全反式维甲酸(ATRA)诱导 NB4、HL-60 和 K562 细胞系中性粒细胞分化簇(CD 标记)标记(CD33、CD97、CD54、CD38、CD18、CD11b、CD44 和 CD71)含量在转录本和蛋白质水平上的变化。转录组分析显示,ATRA 反应细胞株 HL-60 和 NB4 早在添加诱导剂 3 小时后就诱导出了 CD38、CD54、CD11b 和 CD18 标记。24 小时后,在所有细胞系中都能观察到特异性的 CD 标记表达模式。通过流式细胞仪和靶向质谱(MS)对 CD 抗原含量变化的研究也得出了类似的结果。NB4和HL-60细胞系特有的表面标志物(CD38、CD54、CD11b和CD18)的蛋白质组特征反映了ATRA诱导白血病细胞分化为成熟中性粒细胞的不同分子途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
System analysis of surface CD markers during the process of granulocytic differentiation.

Plasma membrane proteins with extracellular-exposed domains are responsible for transduction of extracellular signals into intracellular responses, and their accessibility to therapeutic molecules makes them attractive targets for drug development. In this work, using omics technologies and immunochemical methods, we have studied changes in the content of markers of clusters of differentiation (CD markers) of neutrophils (CD33, CD97, CD54, CD38, CD18, CD11b, CD44, and CD71) at the level of transcripts and proteins in NB4, HL-60 and K562 cell lines, induced by the treatment with all-trans-retinoic acid (ATRA). Transcriptomic analysis revealed the induction of CD38, CD54, CD11b, and CD18 markers as early as 3 h after the addition of the inducer in the ATRA-responsive cell lines HL-60 and NB4. After 24 h, a line-specific expression pattern of CD markers could be observed in all cell lines. Studies of changes in the content of CD antigens by means of flow cytometry and targeted mass spectrometry (MS) gave similar results. The proteomic profile of the surface markers (CD38, CD54, CD11b, and CD18), characteristic of the NB4 and HL-60 lines, reflects different molecular pathways for the implementation of ATRA-induced differentiation of leukemic cells into mature neutrophils.

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来源期刊
Biomeditsinskaya khimiya
Biomeditsinskaya khimiya Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
1.30
自引率
0.00%
发文量
49
期刊介绍: The aim of the Russian-language journal "Biomeditsinskaya Khimiya" (Biomedical Chemistry) is to introduce the latest results obtained by scientists from Russia and other Republics of the Former Soviet Union. The Journal will cover all major areas of Biomedical chemistry, including neurochemistry, clinical chemistry, molecular biology of pathological processes, gene therapy, development of new drugs and their biochemical pharmacology, introduction and advertisement of new (biochemical) methods into experimental and clinical medicine etc. The Journal also publish review articles. All issues of journal usually contain invited reviews. Papers written in Russian contain abstract (in English).
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