体外培养益生菌对改善家禽业健康的生态作用

Q4 Agricultural and Biological Sciences
Olivier Nsenge, B. Mangata, D. Eyul’anki, Emmanuel Makaly
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引用次数: 0

摘要

这项工作的总体目标是从酸奶中分离出有可能用作家禽养殖益生菌的乳酸菌菌株。我们培养了三种酸奶,以观察罗戈萨琼脂基础培养基中是否存在乳酸菌。结果发现,只有一种酸奶(1 号)有乳酸菌生长。我们立即选择了这种酸奶进行进一步培养。随后,分离出乳酸杆菌菌株并在二氧化碳条件下进行强化,然后将其接种到蛋白胨水溶液中,作为接种物喂给实验组家禽。另一组作为对照组。两组家禽(实验组和对照组,每组五只母鸡)都接种了致病性大肠杆菌菌株。结果表明,实验组的家禽体重明显增加(对免疫力有积极影响),并且在孵化期后没有发病(乳酸菌株对大肠杆菌株有抑制作用),而对照组的家禽体重增加少于实验组,并且在孵化期后出现了大肠杆菌病。乳酸杆菌菌株对实验组家禽的积极影响证明,这些微生物通过抑制致病性大肠杆菌菌株的影响,在改善家禽健康方面发挥了生态作用。这为在人类和动物护理中减少抗生素的使用提供了令人欣慰的前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Ecological Role of Probiotics in in vitro Culture for the Improvement of Health in the Poultry Industry
The general objective of this work was to isolate from yoghourts cultured strains of lactobacilli with potential for use as probiotics in poultry farming. Three yoghourts were cultured to see the presence of lactobacilli in the Rogosa agar base culture medium. It was found that only one yoghourt (number 1) showed the growth of lactobacilli. This yoghourt was immediately selected for further cultivation. Afterwards, the Lactobacillus strains were isolated and fortified under CO2 and then inoculated into a solution of peptone water, which constituted inoculums to be administered to an experimental group of poultry. Another group served as controls. Pathogenic strains of Escherichia coli were also administered to both groups (experimental and control, each comprising five hens). The results showed significant weight gain from the experimental group (positive effect on immunity) and freedom from disease after an incubation period (inhibitory effect of the lactobacillus strains on the Escherichia coli strains), whereas the control group showed less weight gain than the experimental group and development of colibacillosis after an incubation period. The positive effects of the Lactobacillus strains observed on the poultry of the experimental group proved the ecological role of these microorganisms in improving the health of the poultry by inhibiting the effects of pathogenic Escherichia coli strains. This suggests reassuring prospects for the reduction of antibiotic use in both human and animal care.
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来源期刊
International Journal Bioautomation
International Journal Bioautomation Agricultural and Biological Sciences-Food Science
CiteScore
1.10
自引率
0.00%
发文量
22
审稿时长
12 weeks
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