平衡激素和基因表达，实现成功生根：洛伐他汀揭示了苹果茎插条中细胞分裂素的抑制作用

IF 3.1 3区农林科学 Q1 HORTICULTURE

Horticulturae Pub Date : 2023-12-15 DOI:10.3390/horticulturae9121341

Sinuo Sun, M. Tahir, Zushu Xie, Pengyan Wei, Jianing Yu, Hangkong Liu, Yinnan He, Xiaoying Ren, Yuanyuan Ma, Jiangping Mao

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引用次数: 0

摘要

不定根（AR）的形成是无性繁殖的关键；然而，细胞分裂素（CK）会阻碍不定根的形成。但细胞分裂素抑制不定根形成的机制尚不清楚。在这项研究中，我们用外源 6-苄基腺嘌呤（6-BA）（1 毫克/升）和洛伐他汀（CK 生物合成抑制剂）（1 毫克/升）处理的 Malus prunifolia var.结果表明，对照组和经 6-BA 处理的插条未能产生 ARs；然而，经洛伐他汀处理的插条在处理 20 天（d）后，通过在多个时间点增加吲哚-3-乙酸（IAA）和降低玉米素核糖甙（ZR）含量，成功产生了少量 ARs。6-BA 处理在大多数时间点诱导了 CK 相关基因的表达，如 MdARR3、MdARR5、MdARR5-2、MdAKH4 和 MdCKX5。然而，洛伐他汀处理过的插条降低了它们的表达，这有利于 AR 的形成。此外，洛伐他汀处理还诱导了包括 MdIAA23、MdARF7 和 MdARF19 在内的辅助素相关基因的表达。与辅助素相关基因一样，一些与根系发育相关的基因（MdWOX5、MdWOX11、MdLB29 和 MdARRO1）也在洛伐他汀处理后得到促进，而 6-BA 和对照插条则抑制了这些基因的表达。总之，与对照组和6-BA处理的插条相比，洛伐他汀处理通过抑制插条内CK的生物合成来支持AR的形成。这项研究为今后研究 CK 生物合成抑制剂与苹果和其他作物不定根的关系奠定了基础。

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Balancing Hormones and Gene Expressions for Rooting Success: Lovastatin Unveils Cytokinin Inhibition in Malus prunifolia var. ringo Apple Stem Cuttings

Adventitious root (AR) formation is the key to asexual reproduction; however, cytokinin (CK) hampers AR formation. But the mechanism by which CK inhibits it is still unknown. In this study, we used Malus prunifolia var. ringo apple stem cuttings that were treated with exogenous 6-benzyl adenine (6-BA) at 1 mg/L and lovastatin (CK biosynthesis inhibitor) at 1 mg/L to compare with control (untreated) cuttings. The results indicated that the control and 6-BA-treated cuttings failed to produce ARs; however, lovastatin-treated cuttings successfully produced a few ARs after 20 days (d) of treatments by increasing indole-3-acetic acid (IAA) and reducing zeatin riboside (ZR) content at several time points. The 6-BA treatment induced the expression of CK-related genes, such as MdARR3, MdARR5, MdARR5-2, MdAKH4, and MdCKX5, at most time points. However, lovastatin-treated cuttings reduced their expression, which favors AR formation. Furthermore, the expression of auxin-related genes, including MdIAA23, MdARF7, and MdARF19, was induced by lovastatin treatment. Like auxin-related genes, several root-development-related genes (MdWOX5, MdWOX11, MdLB29, and MdARRO1) were also promoted in response to lovastatin treatment that were repressed by 6-BA and control cuttings. In conclusion, lovastatin treatment supports AR formation by inhibiting CK biosynthesis inside the cuttings, as compared to the control and 6-BA-treated cuttings. This study laid the foundation for future studies on the relationship of CK biosynthesis inhibitors with adventitious rooting in apples and other crops.

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来源期刊

Horticulturae HORTICULTURE-

CiteScore

3.50

自引率

19.40%

发文量

998

期刊介绍： Horticulturae (ISSN 2311-7524) is an international, multidisciplinary, peer-reviewed, open access journal focusing on all areas and aspects of temperate to tropical horticulture. It publishes original empirical and theoretical research articles, short communications, reviews, and opinion articles. We intend to encourage scientists to publish and communicate their results concerning all branches of horticulture in a timely manner and in an open venue, after being evaluated by the journal editors and randomly selected independent expert reviewers, so that all articles will never be judged in relation to how much they confirm or criticize the opinions of other researchers.