生物信息学研究及谷胱甘肽 S-转移酶基因在鲤鱼(Cyprinus carpio)和褐鳟鱼(Salmo trutta)部分组织中的 mRNA 转录比较

Badrul Islam Elsevar, M. Bayır
{"title":"生物信息学研究及谷胱甘肽 S-转移酶基因在鲤鱼(Cyprinus carpio)和褐鳟鱼(Salmo trutta)部分组织中的 mRNA 转录比较","authors":"Badrul Islam Elsevar, M. Bayır","doi":"10.12714/egejfas.40.4.05","DOIUrl":null,"url":null,"abstract":"Bioinformatics has revolutionized the way we study gene expression and regulation, enabling researchers to analyze large-scale genomic data with unprecedented speed and precision. In this study, we use bioinformatics tools and methods to compare mRNA transcription of glutathione S-transferase (gstr) gene in two different fish species: common carp and brown trout. In this study, liver, intestine, muscle, brain, heart, eye, spleen, gill, kidney, stomach, ovary and testis samples were taken from male and female brown trout and common carp, and total RNA was isolated from each tissue to synthesize cDNA from these tissues. Then, the transcript amounts of the gstr gene were determined by qPCR from all tissue samples. Gene structures, conserved gene synteny design, phyogenetic tree analyzes and similarity-identity ratios with other vertebrates were determined. When the transcriptional differences between male and female tissues for the brown trout gstr gene were examined, it was seen that the intestine, gill, kidney, stomach, muscle and gonads were significantly higher in male fish (p<0.05), but the differences between other tissues were not statistically significant. It has been determined that the highest gene expression was liver (p<0.05) and brain, eye, spleen, kidney, heart and spleen tissues have significantly lower gstr gene expression than other tissues in both male and female in common carp. In addition, the in-silico analysis determined that the brown trout gstr gene shared the highest similarity and identity ratio with rainbow trout, and the common carp gstr gene shared the highest similarity and identity ratio with goldfish.","PeriodicalId":11439,"journal":{"name":"Ege Journal of Fisheries and Aquatic Sciences","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Bioinformatics studies and comparison of mRNA transcription of glutathione S-transferase gene in some tissues of common carp (Cyprinus carpio) and brown trout (Salmo trutta)\",\"authors\":\"Badrul Islam Elsevar, M. Bayır\",\"doi\":\"10.12714/egejfas.40.4.05\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Bioinformatics has revolutionized the way we study gene expression and regulation, enabling researchers to analyze large-scale genomic data with unprecedented speed and precision. In this study, we use bioinformatics tools and methods to compare mRNA transcription of glutathione S-transferase (gstr) gene in two different fish species: common carp and brown trout. In this study, liver, intestine, muscle, brain, heart, eye, spleen, gill, kidney, stomach, ovary and testis samples were taken from male and female brown trout and common carp, and total RNA was isolated from each tissue to synthesize cDNA from these tissues. Then, the transcript amounts of the gstr gene were determined by qPCR from all tissue samples. Gene structures, conserved gene synteny design, phyogenetic tree analyzes and similarity-identity ratios with other vertebrates were determined. When the transcriptional differences between male and female tissues for the brown trout gstr gene were examined, it was seen that the intestine, gill, kidney, stomach, muscle and gonads were significantly higher in male fish (p<0.05), but the differences between other tissues were not statistically significant. It has been determined that the highest gene expression was liver (p<0.05) and brain, eye, spleen, kidney, heart and spleen tissues have significantly lower gstr gene expression than other tissues in both male and female in common carp. In addition, the in-silico analysis determined that the brown trout gstr gene shared the highest similarity and identity ratio with rainbow trout, and the common carp gstr gene shared the highest similarity and identity ratio with goldfish.\",\"PeriodicalId\":11439,\"journal\":{\"name\":\"Ege Journal of Fisheries and Aquatic Sciences\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-12-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Ege Journal of Fisheries and Aquatic Sciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.12714/egejfas.40.4.05\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Ege Journal of Fisheries and Aquatic Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.12714/egejfas.40.4.05","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

生物信息学彻底改变了我们研究基因表达和调控的方式,使研究人员能够以前所未有的速度和精度分析大规模基因组数据。在这项研究中,我们利用生物信息学工具和方法比较了谷胱甘肽 S-转移酶(gstr)基因在两种不同鱼类(鲤鱼和褐鳟)中的 mRNA 转录情况。本研究采集了雌雄褐鳟和鲤鱼的肝、肠、肌肉、脑、心、眼、脾、鳃、肾、胃、卵巢和睾丸样本,并从各组织中分离总 RNA 合成 cDNA。然后,通过 qPCR 方法测定了所有组织样本中 gstr 基因的转录量。确定了基因结构、保守基因同源设计、植物基因树分析以及与其他脊椎动物的相似性-相同性比。在研究褐鳟 gstr 基因在雌雄组织间的转录差异时,发现雄鱼的肠道、鳃、肾、胃、肌肉和性腺的转录差异显著高于雌鱼(p<0.05),但其他组织间的差异无统计学意义。经测定,肝脏的基因表达量最高(p<0.05),脑、眼、脾、肾、心和脾组织的 gstr 基因表达量在雌雄鲤鱼中均明显低于其他组织(p<0.05)。此外,通过分子内分析确定,褐鳟的 gstr 基因与虹鳟的相似度和同一性最高,而鲤鱼的 gstr 基因与金鱼的相似度和同一性最高。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Bioinformatics studies and comparison of mRNA transcription of glutathione S-transferase gene in some tissues of common carp (Cyprinus carpio) and brown trout (Salmo trutta)
Bioinformatics has revolutionized the way we study gene expression and regulation, enabling researchers to analyze large-scale genomic data with unprecedented speed and precision. In this study, we use bioinformatics tools and methods to compare mRNA transcription of glutathione S-transferase (gstr) gene in two different fish species: common carp and brown trout. In this study, liver, intestine, muscle, brain, heart, eye, spleen, gill, kidney, stomach, ovary and testis samples were taken from male and female brown trout and common carp, and total RNA was isolated from each tissue to synthesize cDNA from these tissues. Then, the transcript amounts of the gstr gene were determined by qPCR from all tissue samples. Gene structures, conserved gene synteny design, phyogenetic tree analyzes and similarity-identity ratios with other vertebrates were determined. When the transcriptional differences between male and female tissues for the brown trout gstr gene were examined, it was seen that the intestine, gill, kidney, stomach, muscle and gonads were significantly higher in male fish (p<0.05), but the differences between other tissues were not statistically significant. It has been determined that the highest gene expression was liver (p<0.05) and brain, eye, spleen, kidney, heart and spleen tissues have significantly lower gstr gene expression than other tissues in both male and female in common carp. In addition, the in-silico analysis determined that the brown trout gstr gene shared the highest similarity and identity ratio with rainbow trout, and the common carp gstr gene shared the highest similarity and identity ratio with goldfish.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信