印楝（Azadirachta indica）叶提取物负载的磷酸钙纳米粒子对大鼠实验诱导的乳腺癌发生的抗癌潜力评估

Q4 Pharmacology, Toxicology and Pharmaceutics

Toxicology International Pub Date : 2023-12-11 DOI:10.18311/ti/2023/v30i4/35228

S. Chavhan, C. Balachandran, A. Nambi, G. D. Raj, S. Vairamuthu

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引用次数: 0

摘要

考虑到替代医学在缓解肿瘤方面的需求，以及利用纳米技术进一步发挥草药/天然产品的作用，本研究旨在评估楝树叶提取物（NE）负载的磷酸钙纳米颗粒（NE-CaNP）对 7，12-二甲基苯并蒽（DMBA）诱导的雌性 Sprague-Dawley 大鼠乳腺肿瘤的抗癌潜力。从超微结构上看，NE-CaNP 为光滑的球形，有结块倾向，分布均匀。NE-CaNP 的平均直径为 231.4 ± 89.2 nm，zeta 电位为 -31.3mV。CaNP 的平均耦合效率为 90-91%。实验包括对照组、NE-CaNP 对照组、DMBA 组、DMBA+NE 组和 DMBA+NE-CaNP 组。与 DMBA 组相比，DMBA+NE 组和 DMBA+NE-CaNP 组的乳腺肿瘤发生平均潜伏期显著延长（P ≤ 0.05）。DMBA+NE-CaNP组的平均潜伏期明显高于DMBA+NE组（P≤0.05）。DMBA+NE-CaNP 组的平均肿瘤频率、体积和重量明显降低（P ≤ 0.05）。从组织病理学角度看，DMBA+NE-CaNP 组大鼠良性病变数量最多（47.54%）。与 DMBA 组相比，DMBA+NE 组和 DMBA+NE-CaNP 组恶性肿瘤的相对减少率分别为 42.86% 和 54.29%。总之，与单独使用印楝提取物治疗的大鼠相比，印楝叶提取物负载磷酸钙纳米粒子具有更好的抗癌潜力，可显著降低雌性 Sprague-Dawley 大鼠 DMBA 诱导的乳腺肿瘤的发病率、频率、重量、体积、恶性程度，并延长肿瘤潜伏期。本研究结果表明，印楝叶提取物负载磷酸钙纳米粒子（NE-CaNP）在减少肿瘤负荷和恶性程度方面具有巨大的抗癌潜力。

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Assessment of Anti-Carcinogenic Potential of Neem (Azadirachta indica) Leaf Extract Loaded Calcium Phosphate Nanoparticles against Experimentally Induced Mammary Carcinogenesis in Rats

Considering the need for alternative medicine in alleviation of tumors and use of nanotechnology in furthering the action of herbal/natural products, the present study was designed to evaluate the anti-carcinogenic potential of Neem leaf Extract (NE) loaded calcium phosphate nanoparticles (NE-CaNP) on 7, 12-Dimethylbenzanthracene (DMBA) induced mammary tumors in female Sprague-Dawley rats. Ultrastructurally, the NE-CaNP were smooth, spherical with a tendency to agglomerate and evenly distributed. The NE-CaNP had a mean diameter of 231.4 ± 89.2 nm and zeta potential of -31.3mV. The mean coupling efficiency of CaNP was 90-91 %. The experimental trial consisted of control, NE-CaNP control, DMBA, DMBA+NE and DMBA+NE-CaNP groups. The mean latency periods for occurrence of mammary tumor were significantly (P ≤ 0.05) increased in the DMBA+NE and DMBA+NE-CaNP groups compared to the DMBA group. The mean latency period in the DMBA+NE-CaNP group was significantly (P ≤ 0.05) higher than the DMBA+NE group. The mean tumor frequency, volume and weight were significantly (P ≤ 0.05) decreased in the DMBA+NE-CaNP group. Histopathologically, the number of benign lesions was found highest (47.54%) in DMBA+NE-CaNP group rats. The relative percent reduction in malignancy as compared to the DMBA group was 42.86% and 54.29% in the DMBA+NE and DMBA+NE-CaNP groups respectively. In conclusion, the neem leaf extract loaded calcium phosphate nanoparticles were found to have better anti-carcinogenic potential by significantly reducing the incidence, frequency, weight, volume, malignancy and increased the tumor latency period of DMBA induced mammary tumors in female Sprague-Dawley rats as compared to rats treated with neem extract alone. Findings of the present study suggested that the neem leaf extract loaded calcium phosphate nanoparticles (NE-CaNP) has immense anticancer potential in terms of reduction in tumor burden and malignancy.

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来源期刊

Toxicology International Environmental Science-Health, Toxicology and Mutagenesis

CiteScore

0.60

自引率

0.00%

发文量

期刊介绍： Toxicology International is a peer-reviewed International Research Journal published bi-annually by the Society of Toxicology, India. The Journal is concerned with various disciplines of Toxicology including man, animals, plants and environment and publishes research, review and general articles besides opinions, comments, news-highlights and letters to editor.