C-SPAM：具有光激活分子的开放源时间分辨标本玻璃化装置。

IF 2.9 2区材料科学 Q2 CHEMISTRY, MULTIDISCIPLINARY

IUCrJ Pub Date : 2024-01-01 DOI:10.1107/S2052252523010308

Alejandra Montaño Romero , Calli Bonin , Edward C. Twomey , F. Sun (Editor)

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引用次数: 0

摘要

冷冻电镜（cryo-EM）可在体外和原位确定分子结构。样品制备是冷冻电镜的一大障碍。典型的样品制备比生物过程慢几个数量级。时间分辨冷冻电子显微镜（TR-cryo-EM）可以捕捉短寿命状态。这里介绍的是利用光激活分子（C-SPAM）进行冷冻电镜样品制备，这是一种用于 TR 冷冻电镜的开源光化学耦合设备，可实现毫秒级分辨率和可调时间尺度，应用于广泛的生物领域。

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C-SPAM: an open-source time-resolved specimen vitrification device with light-activated molecules

An open-source platform for light-coupled cryo-electron microscopy specimen preparation is presented.

Molecular structures can be determined in vitro and in situ with cryo-electron microscopy (cryo-EM). Specimen preparation is a major obstacle in cryo-EM. Typical sample preparation is orders of magnitude slower than biological processes. Time-resolved cryo-EM (TR-cryo-EM) can capture short-lived states. Here, Cryo-EM sample preparation with light-activated molecules (C-SPAM) is presented, an open-source, photochemistry-coupled device for TR-cryo-EM that enables millisecond resolution and tunable timescales across broad biological applications.

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来源期刊

IUCrJ CHEMISTRY, MULTIDISCIPLINARYCRYSTALLOGRAPH-CRYSTALLOGRAPHY

CiteScore

7.50

自引率

5.10%

发文量

审稿时长

10 weeks

期刊介绍： IUCrJ is a new fully open-access peer-reviewed journal from the International Union of Crystallography (IUCr). The journal will publish high-profile articles on all aspects of the sciences and technologies supported by the IUCr via its commissions, including emerging fields where structural results underpin the science reported in the article. Our aim is to make IUCrJ the natural home for high-quality structural science results. Chemists, biologists, physicists and material scientists will be actively encouraged to report their structural studies in IUCrJ.