Meng Yu, Yeyi Yang, Juan Zhang, Rui Liu, Lihua Huang, Jiping Wu, Zhijuan Kang, Jin Zhou, Zuocheng Yang
{"title":"中国儿童 TRAF1/C5 基因多态性与 IgA 血管炎的关系","authors":"Meng Yu, Yeyi Yang, Juan Zhang, Rui Liu, Lihua Huang, Jiping Wu, Zhijuan Kang, Jin Zhou, Zuocheng Yang","doi":"10.1080/08820139.2023.2295477","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To investigate the association between loci rs3761847 and rs10818488 of tumor necrosis factor receptor-associated factor 1/complement C5 (<i>TRAF1/C5</i>) gene and the susceptibility to IgAV.</p><p><strong>Methods: </strong>100 blood samples of children with IgAV and 100 blood samples of healthy children were collected from the Third Xiangya Hospital of Central South University from June 2017 to June 2019. The target gene fragment was amplified by polymerase chain reaction (PCR), and the single nucleic acid gene polymorphism of the gene loci was detected by PCR sequencing based typing technique. The association between gene polymorphism of each locus and susceptibility to IgAV was analyzed.</p><p><strong>Results: </strong>There were significant differences in both genotype (<i>P</i> < .05) and allele frequencies （<i>P</i> < .05) of rs3761847 of <i>TRAF1/C5</i> gene between the IgAV group and the control group.Besides, the risks of developing IgAV in children with the TT genotype was 0.495 times and in children with the C allele was 1.627 times of that in children with other genotypes and alleles, respectively (<i>P</i> < .05). For IgAV patients, renal involvement risk in children with CC genotype was 5.859 times of that in children with other genotypes (<i>P</i> < .05). There were no significant differences in genotype (<i>P</i> > .05) and allele frequencies (<i>P</i> > .05) of rs10818488 of <i>TRAF1/C5</i> gene between the IgAV group and the control group. IgAV patients with TT genotype had a 3.2 times higher risk of renal involvement than those with other genotypes (<i>P</i> < .05).</p><p><strong>Conclusions: </strong>There is an association between locus rs3761847 of <i>TRAF1/C5</i> gene single nucleotide polymorphisms and susceptibility to IgAV. The T allele at locus rs3761847 of <i>TRAF1/C5</i> gene may be a protective factor for IgAV. The C allele at locus rs3761847 and the T allele at locus rs10818488 of <i>TRAF1/C5</i> gene may be associated with kidney injury in IgAV.</p>","PeriodicalId":13387,"journal":{"name":"Immunological Investigations","volume":" ","pages":"281-293"},"PeriodicalIF":2.9000,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Association between <i>TRAF1/C5</i> Gene Polymorphisms and IgA Vasculitis in Chinese Children.\",\"authors\":\"Meng Yu, Yeyi Yang, Juan Zhang, Rui Liu, Lihua Huang, Jiping Wu, Zhijuan Kang, Jin Zhou, Zuocheng Yang\",\"doi\":\"10.1080/08820139.2023.2295477\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To investigate the association between loci rs3761847 and rs10818488 of tumor necrosis factor receptor-associated factor 1/complement C5 (<i>TRAF1/C5</i>) gene and the susceptibility to IgAV.</p><p><strong>Methods: </strong>100 blood samples of children with IgAV and 100 blood samples of healthy children were collected from the Third Xiangya Hospital of Central South University from June 2017 to June 2019. The target gene fragment was amplified by polymerase chain reaction (PCR), and the single nucleic acid gene polymorphism of the gene loci was detected by PCR sequencing based typing technique. The association between gene polymorphism of each locus and susceptibility to IgAV was analyzed.</p><p><strong>Results: </strong>There were significant differences in both genotype (<i>P</i> < .05) and allele frequencies （<i>P</i> < .05) of rs3761847 of <i>TRAF1/C5</i> gene between the IgAV group and the control group.Besides, the risks of developing IgAV in children with the TT genotype was 0.495 times and in children with the C allele was 1.627 times of that in children with other genotypes and alleles, respectively (<i>P</i> < .05). For IgAV patients, renal involvement risk in children with CC genotype was 5.859 times of that in children with other genotypes (<i>P</i> < .05). There were no significant differences in genotype (<i>P</i> > .05) and allele frequencies (<i>P</i> > .05) of rs10818488 of <i>TRAF1/C5</i> gene between the IgAV group and the control group. IgAV patients with TT genotype had a 3.2 times higher risk of renal involvement than those with other genotypes (<i>P</i> < .05).</p><p><strong>Conclusions: </strong>There is an association between locus rs3761847 of <i>TRAF1/C5</i> gene single nucleotide polymorphisms and susceptibility to IgAV. The T allele at locus rs3761847 of <i>TRAF1/C5</i> gene may be a protective factor for IgAV. The C allele at locus rs3761847 and the T allele at locus rs10818488 of <i>TRAF1/C5</i> gene may be associated with kidney injury in IgAV.</p>\",\"PeriodicalId\":13387,\"journal\":{\"name\":\"Immunological Investigations\",\"volume\":\" \",\"pages\":\"281-293\"},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2024-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Immunological Investigations\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1080/08820139.2023.2295477\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2023/12/20 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"IMMUNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Immunological Investigations","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/08820139.2023.2295477","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/12/20 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
目的研究肿瘤坏死因子受体相关因子1/补体C5（TRAF1/C5）基因位点rs3761847和rs10818488与IgAV易感性的相关性。方法：2017年6月至2019年6月在中南大学湘雅三医院采集100例IgAV患儿血样和100例健康儿童血样。采用聚合酶链反应（PCR）扩增目的基因片段，基于PCR测序的分型技术检测基因位点的单核酸基因多态性。分析了各基因位点的基因多态性与 IgAV 易感性之间的关联：此外，IgAV 组与对照组之间 TRAF1/C5 基因 rs10818488 的基因型（P P > .05）和等位基因频率（P > .05）分别为 TT 基因型儿童患 IgAV 风险的 0.495 倍和 C 等位基因儿童患 IgAV 风险的 1.627 倍。TT基因型的IgAV患者肾脏受累的风险是其他基因型患者的3.2倍（P 结论：TT基因型的IgAV患者肾脏受累的风险是其他基因型患者的3.2倍：TRAF1/C5 基因位点 rs3761847 单核苷酸多态性与 IgAV 易感性之间存在关联。TRAF1/C5 基因位点 rs3761847 的 T 等位基因可能是 IgAV 的保护因素。TRAF1/C5 基因位点 rs3761847 的 C 等位基因和位点 rs10818488 的 T 等位基因可能与 IgAV 肾损伤有关。
Association between TRAF1/C5 Gene Polymorphisms and IgA Vasculitis in Chinese Children.
Objective: To investigate the association between loci rs3761847 and rs10818488 of tumor necrosis factor receptor-associated factor 1/complement C5 (TRAF1/C5) gene and the susceptibility to IgAV.
Methods: 100 blood samples of children with IgAV and 100 blood samples of healthy children were collected from the Third Xiangya Hospital of Central South University from June 2017 to June 2019. The target gene fragment was amplified by polymerase chain reaction (PCR), and the single nucleic acid gene polymorphism of the gene loci was detected by PCR sequencing based typing technique. The association between gene polymorphism of each locus and susceptibility to IgAV was analyzed.
Results: There were significant differences in both genotype (P < .05) and allele frequencies （P < .05) of rs3761847 of TRAF1/C5 gene between the IgAV group and the control group.Besides, the risks of developing IgAV in children with the TT genotype was 0.495 times and in children with the C allele was 1.627 times of that in children with other genotypes and alleles, respectively (P < .05). For IgAV patients, renal involvement risk in children with CC genotype was 5.859 times of that in children with other genotypes (P < .05). There were no significant differences in genotype (P > .05) and allele frequencies (P > .05) of rs10818488 of TRAF1/C5 gene between the IgAV group and the control group. IgAV patients with TT genotype had a 3.2 times higher risk of renal involvement than those with other genotypes (P < .05).
Conclusions: There is an association between locus rs3761847 of TRAF1/C5 gene single nucleotide polymorphisms and susceptibility to IgAV. The T allele at locus rs3761847 of TRAF1/C5 gene may be a protective factor for IgAV. The C allele at locus rs3761847 and the T allele at locus rs10818488 of TRAF1/C5 gene may be associated with kidney injury in IgAV.
期刊介绍：
Disseminating immunological developments on a worldwide basis, Immunological Investigations encompasses all facets of fundamental and applied immunology, including immunohematology and the study of allergies. This journal provides information presented in the form of original research articles and book reviews, giving a truly in-depth examination of the latest advances in molecular and cellular immunology.
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