实验诱导的动物模型胆脂瘤对血卟啉的摄取。

G P Teatini, C Perria, G Iori, F Meloni, F Tanda
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引用次数: 5

摘要

光动力疗法是基于卟啉,特别是血卟啉(HP)在暴露于适当波长和强度的光下时产生细胞毒性因子。HP的摄取在有丝分裂指数高的组织中显著增加。虽然胆脂瘤不是恶性肿瘤,但我们的工作假设是,它们的高脂含量可能导致它们与HP表现出显著的亲和力,HP通常通过脂蛋白在血液中携带。采用宾根法(缝合耳道)诱导兔胆脂瘤。分别于静脉注射HP (5 mg/kg)后1、3、6、12和24 h,于30-40天后处死动物。标本分为两部分,一部分用于组织学检查,另一部分用于生化研究。后者显示,HP在实验性胆脂瘤中积累,在3小时后达到最大摄取,然后水平逐渐下降,尽管速度低于肝脏,但即使在24小时后仍保持相当高的水平。这些结果表明,光动力治疗胆脂瘤在我们的动物模型中应该是可行的,尽管这种治疗在人体中仍是推测性的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Hematoporphyrin uptake by experimentally induced cholesteatomas in an animal model.

Photodynamic therapy is based on the production of a cytotoxic factor by porphyrins, particularly hematoporphyrin (HP), when exposed to light of a suitable wavelength and intensity. The uptake of HP is notably large in tissues with a high mitotic index. Although cholesteatomas are not malignant tumors, our working hypothesis was that their high lipid content might result in their exhibiting a remarkable affinity to HP, which is normally carried in the blood by lipoproteins. Cholesteatomas were induced in rabbits using the Tübingen procedure (closure of the auditory canal by sutures). Animals were killed 30-40 days later at intervals of 1, 3, 6, 12, and 24 h following intravenous HP administration (5 mg/kg). Specimens were divided into two portions, one for histological examination and the other for biochemical study. The latter revealed that HP accumulates in experimental cholesteatomas, with a maximum uptake after 3 h. The level then gradually decreases, although at a lower rate than in the liver, but remains considerably high even after 24 h. These results suggest that the photodynamic treatment of cholesteatomas should be feasible in our animal model, although such treatment is still speculative in man.

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