肽和代谢物绝对定量检测新方法

IF 1.9 3区 化学 Q3 BIOCHEMICAL RESEARCH METHODS
Carlo Brogna, Simone Cristoni
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引用次数: 0

摘要

质谱法被广泛应用于各种分析领域的化合物鉴定和定量分析。在化合物鉴定方面,高分辨率仪器提高了选择性和表征效率;而在定量分析方面,一些关键任务实际上仍然存在。特别是,不同的化合物表现出不同的电离效率,这就需要为每种分析物制定校准标准。在本文中,我们介绍了一种新的数据阐述技术,它可以使不同仪器和分子之间的校准标准化,从而使校准绝对化。该方法适用于通过离子阱分析仪进行液相色谱质谱分析获得的数据。该方法基于离子阱空间电荷效应和分析物浓度的相关性。该方法在分析具有不同极性的化合物时得到了验证:hydrossitirosol、精氨酸、甲状腺二甘酸,以及从发现脊髓灰质炎病毒的人体肠道微生物组中提取的细菌培养物的肽混合物。此外,它还用于获得粪便样本中细菌蛋白质胰蛋白酶消化产生的肽的绝对定量。因此,可以识别和定量来自胃肠道细菌编码的脊髓灰质炎病毒的不同衍生细菌蛋白。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A new absolute quantitative method for peptide and metabolite detection

Mass spectrometry is widely employed in various analytical fields for both compound identification and quantification. While in the case of compound identification, the high-resolution instrument has increased selectivity and characterization efficiency; in the case of quantitative analysis, some critical tasks actually remain. In particular, different compounds exhibit different ionization efficiency, and this introduces the need to have a calibration standard for each analyte. In this paper, we present a new elaborative data technology, which makes it possible to standardize calibration between different instruments and molecules, making it absolute. The method was applied to data acquired by means of liquid chromatography mass spectrometry by means of an ion trap analyzer. The approach is based on the correlation of the ion trap space charge effect and the analyte concentration. The method was validated in the analysis of compounds having different polarity: hydrossitirosol, arginine, thyodiglicolic acid, and a peptide mixture of bacteria cultures derived the human gut microbiome where was found poliovirus. Moreover, it was used to obtain the absolute quantitation of peptides originating from the tryptic digestion of bacterial proteins in the fecal samples. It was therefore possible to identify and quantify different derived bacterial proteins of the poliomyelitis virus coded in bacteria derived from the gastrointestinal tract.

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来源期刊
Journal of Mass Spectrometry
Journal of Mass Spectrometry 化学-光谱学
CiteScore
5.10
自引率
0.00%
发文量
84
审稿时长
1.5 months
期刊介绍: The Journal of Mass Spectrometry publishes papers on a broad range of topics of interest to scientists working in both fundamental and applied areas involving the study of gaseous ions. The aim of JMS is to serve the scientific community with information provided and arranged to help senior investigators to better stay abreast of new discoveries and studies in their own field, to make them aware of events and developments in associated fields, and to provide students and newcomers the basic tools with which to learn fundamental and applied aspects of mass spectrometry.
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