Harshita Sonarthi, Sathish Kumar M H, Champalli S Rajani, Ankur Sharma, Arumugam Kumaresan, Latha Sabikhi
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Production of DPP-IV inhibitory peptides-rich beta casein hydrolysates from milk of Gir (Bos indicus) cow and their evaluation for potential antidiabetic effect through in vitro assay
An attempt was made to produce hydrolysates from β-casein isolated from milk of Gir cow (Bos indicus species) using pepsin, trypsin, protease from Aspergillus oryzae (AOP) and proteinase-K. The AOP-treated hydrolysates exhibited highest DPP-IV inhibition (50.41 ± 1.44%) after 10 h hydrolysis at 4% E:S ratio. The resultant hydrolysates were characterised using RP-HPLC and LC–MS/MS and their peptides sequences identified. The efficacy of the low molecular weight hydrolysates (3 kDa permeate) was assessed through MIN6 cell line model at different doses. The dose of 30 mg/mL enhanced insulin production by ~51% and reduced the apoptosis by ~84%. The developed β-casein hydrolysates could be used as a dietary supplement to manage diabetes effectively.
期刊介绍：
The International Journal of Dairy Technology ranks highly among the leading dairy journals published worldwide, and is the flagship of the Society. As indicated in its title, the journal is international in scope.
Published quarterly, International Journal of Dairy Technology contains original papers and review articles covering topics that are at the interface between fundamental dairy research and the practical technological challenges facing the modern dairy industry worldwide. Topics addressed span the full range of dairy technologies, the production of diverse dairy products across the world and the development of dairy ingredients for food applications.
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