C. Biazus-Dalcin , T.C.M. Sincero , C.P. Zamparette , D.C. Tartari , S. de Souza , T.L. Silva , A. Tomazoni , P.K. Rocha
{"title":"降低无针连接器鲍曼不动杆菌 blaOXA-23 感染率的消毒程序的效果：一项体外研究","authors":"C. Biazus-Dalcin , T.C.M. Sincero , C.P. Zamparette , D.C. Tartari , S. de Souza , T.L. Silva , A. Tomazoni , P.K. Rocha","doi":"10.1016/j.infpip.2023.100328","DOIUrl":null,"url":null,"abstract":"<div><h3>Aim</h3><p>This study aimed to verify the efficacy of disinfection procedures to reduce <em>Acinetobacter baumannii bla</em><sub>OXA-23</sub> bacterial load in needleless connectors that had been experimentally contaminated.</p></div><div><h3>Methods</h3><p>Two-way intermediate extender's hub and needle-free valve were contaminated with <em>Acinetobacter baumannii bla</em><sub>OXA-23</sub>. To disinfect them, the following procedures were carried out: sterile gauze with 70% ethanol, sterile gauze with Incidin®, and 70% isopropyl alcohol single-use cap, with eight times friction for 10 s, followed by 5 s drying time. The statistical tests Kruskal–Wallis and post-hoc Conover were performed using MedCalc®.</p></div><div><h3>Results</h3><p>A total of 82 experiments were conducted. All tested disinfection procedures were efficacious in reducing the A. <em>baumannii bla</em><sub>OXA-23</sub> load. The 70% IPA single-use cap was found to be the best method for disinfecting the two-way intermediate extender's hub (87.28%), while all the methods were efficacious for the disinfection of the needle-free valve (more than 90%). During the inoculation period, <em>A. baumannii bla</em><sub>OXA-23</sub> showed less adherence to the needle-free valve during the inoculation period, probably due to the device's design.</p></div><div><h3>Conclusion</h3><p>The three tested disinfection procedures using sterile gauze with 70% ethanol, sterile gauze with Incidin®, and 70% IPA single-use cap were found to be efficacious in reducing the bacterial load of <em>A. baumanni bla</em><sub><em>OXA-23</em></sub> in needleless connectors. Proper disinfection of needleless connectors is a crucial nursing practice to prevent bloodstream infections, as it significantly reduces the bacterial load present in the device.</p></div>","PeriodicalId":33492,"journal":{"name":"Infection Prevention in Practice","volume":"6 1","pages":"Article 100328"},"PeriodicalIF":1.8000,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590088923000616/pdfft?md5=61d8e715701963a973546981466696b6&pid=1-s2.0-S2590088923000616-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Efficacy of disinfection procedures to reduce Acinetobacter baumanii blaOXA-23 contamination rate of needleless connectors: an in-vitro study\",\"authors\":\"C. Biazus-Dalcin , T.C.M. Sincero , C.P. Zamparette , D.C. Tartari , S. de Souza , T.L. Silva , A. Tomazoni , P.K. Rocha\",\"doi\":\"10.1016/j.infpip.2023.100328\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Aim</h3><p>This study aimed to verify the efficacy of disinfection procedures to reduce <em>Acinetobacter baumannii bla</em><sub>OXA-23</sub> bacterial load in needleless connectors that had been experimentally contaminated.</p></div><div><h3>Methods</h3><p>Two-way intermediate extender's hub and needle-free valve were contaminated with <em>Acinetobacter baumannii bla</em><sub>OXA-23</sub>. To disinfect them, the following procedures were carried out: sterile gauze with 70% ethanol, sterile gauze with Incidin®, and 70% isopropyl alcohol single-use cap, with eight times friction for 10 s, followed by 5 s drying time. The statistical tests Kruskal–Wallis and post-hoc Conover were performed using MedCalc®.</p></div><div><h3>Results</h3><p>A total of 82 experiments were conducted. All tested disinfection procedures were efficacious in reducing the A. <em>baumannii bla</em><sub>OXA-23</sub> load. The 70% IPA single-use cap was found to be the best method for disinfecting the two-way intermediate extender's hub (87.28%), while all the methods were efficacious for the disinfection of the needle-free valve (more than 90%). During the inoculation period, <em>A. baumannii bla</em><sub>OXA-23</sub> showed less adherence to the needle-free valve during the inoculation period, probably due to the device's design.</p></div><div><h3>Conclusion</h3><p>The three tested disinfection procedures using sterile gauze with 70% ethanol, sterile gauze with Incidin®, and 70% IPA single-use cap were found to be efficacious in reducing the bacterial load of <em>A. baumanni bla</em><sub><em>OXA-23</em></sub> in needleless connectors. Proper disinfection of needleless connectors is a crucial nursing practice to prevent bloodstream infections, as it significantly reduces the bacterial load present in the device.</p></div>\",\"PeriodicalId\":33492,\"journal\":{\"name\":\"Infection Prevention in Practice\",\"volume\":\"6 1\",\"pages\":\"Article 100328\"},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2023-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2590088923000616/pdfft?md5=61d8e715701963a973546981466696b6&pid=1-s2.0-S2590088923000616-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Infection Prevention in Practice\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2590088923000616\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Infection Prevention in Practice","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2590088923000616","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
Efficacy of disinfection procedures to reduce Acinetobacter baumanii blaOXA-23 contamination rate of needleless connectors: an in-vitro study
Aim
This study aimed to verify the efficacy of disinfection procedures to reduce Acinetobacter baumannii blaOXA-23 bacterial load in needleless connectors that had been experimentally contaminated.
Methods
Two-way intermediate extender's hub and needle-free valve were contaminated with Acinetobacter baumannii blaOXA-23. To disinfect them, the following procedures were carried out: sterile gauze with 70% ethanol, sterile gauze with Incidin®, and 70% isopropyl alcohol single-use cap, with eight times friction for 10 s, followed by 5 s drying time. The statistical tests Kruskal–Wallis and post-hoc Conover were performed using MedCalc®.
Results
A total of 82 experiments were conducted. All tested disinfection procedures were efficacious in reducing the A. baumannii blaOXA-23 load. The 70% IPA single-use cap was found to be the best method for disinfecting the two-way intermediate extender's hub (87.28%), while all the methods were efficacious for the disinfection of the needle-free valve (more than 90%). During the inoculation period, A. baumannii blaOXA-23 showed less adherence to the needle-free valve during the inoculation period, probably due to the device's design.
Conclusion
The three tested disinfection procedures using sterile gauze with 70% ethanol, sterile gauze with Incidin®, and 70% IPA single-use cap were found to be efficacious in reducing the bacterial load of A. baumanni blaOXA-23 in needleless connectors. Proper disinfection of needleless connectors is a crucial nursing practice to prevent bloodstream infections, as it significantly reduces the bacterial load present in the device.
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