Efficacy of 2-(p-tolylamino)acetohydrazide and its Co(II), Ni(II) Complexes on the Shell of Eobania vermiculata under Laboratory Conditions

Two new divalent metal constructions were synthesized upon chelation reactions between 2-( p - tolylamino)acetohydrazide ligand (HL) and Co(II), Ni(II) chloride salts. The structure of achieved compounds was identified by conducting a series of spectral and physicochemical techniques. A mixture of distilled water and DMSO was used for preparing four different concentrations of the tested compounds; (50, 100, 200 and 300 ppm), which were then used in treating Eobania vermiculata under laboratory conditions. The thin-layer film technique was utilized for performing the in vitro treatments. The sub-lethal concentrations; (LC 25 and LC 50 ) values were estimated. The investigated chemical compounds displayed remarkable toxicological effects against the screened brown garden snails. Eobania vermiculata species showed the highest sensitivity level toward Ni(II) complex (LC 50 = 53.55 ppm). However, the (HL) ligand displayed a higher toxicity against Eobania vermiculata than Co(II) complex (LC 50 = 87.92 and 95.59 ppm, respectively). The investigated land snails were treated in vitro with the LC 25 concentration of the obtained compounds for 7 days to evaluate their efficacy on the shell of those species. The level of some elements composing the snail shell; calcium, magnesium, phosphorus and potassium, was assessed. The obtained data emphasized that the tested compounds caused a considerable decrease in the level of estimated shell elements.