Arachis L.属中的天然转基因生物

Q3 Agricultural and Biological Sciences

Ecological genetics Pub Date : 2023-12-04 DOI:10.17816/ecogen568618

Olesja D. Bogomaz, V. D. Bemova, T. Matveeva

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引用次数: 0

摘要

栽培花生是一种异源四倍体物种，它接受了duranensis和A. ipaensis的A和B基因组。由于水平转移，在两个基因组中都发现了农杆菌黄瓜合成酶基因的同源物[1]。这些序列在祖先种和栽培花生中都有发现。除此之外，天然转基因作物还有monticola和A.窄乳草。目前尚不清楚天然转基因生物在花生属中有多普遍。本研究的目的是在花生属中寻找天然转基因生物，并分析天然转基因基因在研究种中的多态性。方法:基于SRA NCBI数据库中汇总的NGS数据，采用bwa[2]、GATK[3]和samtools[4]软件包对不同花生品种进行基因测序。结果:我们在不同品种的花生基因组中发现了黄瓜合酶基因的同源物，包括:appressipila、A. batizocoi、A. cardenasii、A. correntina、A. diogoi、A. duranensis、A. glandulifera、A. helodes、A. hoehnei、A. ipaensis、A. macedoi、A. magna、A. monticola、A. paraguariensis、A. pintoi、A. pusilla、A. rigonii、A.窄带、A.狭窄、A.窄尾、A. trinitensis、A. valida、A. villosa，并分析了该基因在栽培花生中的种内变异性。在研究的22个物种中，有16个物种的基因是全长的。该报告将考虑在花生系统发育研究中使用黄瓜合成酶基因的可能性。结论:目前天然转基因花生属植物共有23种。

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Natural GMOs inside the genus Arachis L.

Cultivated peanut is an allotetraploid species that received the A and B genomes from Arachis duranensis and A. ipaensis. Homologs of the agrobacterial cucumopine synthase gene were previously found in both genomes as a result of horizontal transfer [1]. These sequences are found both in ancestral species and in cultivated peanuts. In addition to them, natural GMOs are A. monticola and A. stenosperma. How widespread natural GMOs are within the genus Arachis is currently unknown. The aim of our study was to search for natural GMOs within the genus Arachis and to analyze the polymorphism of natural transgenes out the studied species. METHODS: Gene sequencing for various Arachis species was determined using the bwa [2], GATK [3] and samtools [4] packages based on NGS data aggregated in the SRA NCBI database. RESULTS: We have found homologues of the cucumopine synthase gene in the genomes of A. appressipila, A. batizocoi, A. cardenasii, A. correntina, A. diogoi, A. duranensis, A. glandulifera, A. helodes, A. hoehnei, A. ipaensis, A. macedoi, A. magna, A. monticola, A. paraguariensis, A. pintoi, A. pusilla, A. rigonii, A. stenophylla, A. stenosperma, A. trinitensis, A. valida, A. villosa, and also characterized the intraspecific variability of the gene in cultivated peanuts. In 16 of the 22 species studied, the gene is full-length. The report will consider the possibility of using the cucumopine synthase gene in peanut phylogenetic studies. CONCLUSION: The list of species of natural GMOs within the genus Arachis today includes 23 species.

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来源期刊

Ecological genetics Environmental Science-Ecology

CiteScore

0.90

自引率

0.00%

发文量

期刊介绍： The journal Ecological genetics is an international journal which accepts for consideration original manuscripts that reflect the results of field and experimental studies, and fundamental research of broad conceptual and/or comparative context corresponding to the profile of the Journal. Once a year, the editorial Board reviews and, if necessary, corrects the rules for authors and the journal rubrics.