海洋微藻硫酸化多糖的物理化学和微观结构特征

Analytica Pub Date : 2023-12-05 DOI:10.3390/analytica4040036
Diana Fimbres-Olivarría, J. Marquez-Escalante, K. Martínez-Robinson, Valeria Miranda-Arizmendi, Y. D. Anda-Flores, A. Rascón-Chu, F. Brown‐Bojorquez, E. Carvajal‐Millan
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引用次数: 0

摘要

海藻是一种有价值的多糖来源。然而,关于从微藻中提取硫酸多糖的信息是有限的。Navicula sp.是一种存在于Cortez海的微藻,人们对其多糖的性质知之甚少。研究了Navicula sp.硫酸酸化多糖(NSP)的理化性质和微观结构特征。NSP的傅里叶变换红外光谱显示出不同的波段(1225和820 cm−1,分别分配给S-O和C-O-S拉伸),证实了分子的身份。NSP记录的分子量、特性粘度、旋转半径和流体动力半径分别为1650 kDa、197 mL/g、61 nm和36 nm。zeta电位、电泳迁移率、电导率和扩散系数分别为−5.8 mV、−0.45µm cm/s V、0.70 mS/cm和2.9 × 10−9 cm2/s。NSP的特征比和持续长度分别为4.2和1.3 nm,表明其为非刚性多糖链构象。Mark-Houwink-Sakurada α和K常数分别为0.5和1.67 × 10−1,表明其为分子随机线圈结构。NSP扫描电镜显示其表面粗糙且多孔。了解这些多糖的物理化学和微观结构特征可以作为阐明其结构-功能关系的起点,作为先进生物材料设计的有价值的工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Physicochemical and Microstructural Characteristics of Sulfated Polysaccharide from Marine Microalga
Marine algae are a valuable source of polysaccharides. However, the information available on sulfated polysaccharides from microalgae is limited. Navicula sp. is a microalga present in the Sea of Cortez, of which little is known regarding their polysaccharides’ properties. This study investigated the physicochemical and microstructural characteristics of Navicula sp. sulfated polysaccharide (NSP). The Fourier transform infrared spectrum of NSP showed distinctive bands (1225 and 820 cm−1, assigned to S–O and C–O–S stretching, respectively), confirming the molecular identity. NSP registered molecular weight, intrinsic viscosity, a radius of gyration, and a hydrodynamic radius of 1650 kDa, 197 mL/g, 61 nm, and 36 nm, respectively. The zeta potential, electrophoretic mobility, conductivity, and diffusion coefficient of the molecule were −5.8 mV, −0.45 µm cm/s V, 0.70 mS/cm, and 2.9 × 10−9 cm2/s, respectively. The characteristic ratio and persistence length calculated for NSP were 4.2 and 1.3 nm, suggesting a nonstiff polysaccharide chain conformation. The Mark–Houwink–Sakurada α and K constants were 0.5 and 1.67 × 10−1, respectively, indicating a molecular random coil structure. NSP scanning electron microscopy revealed a rough and porous surface. Knowing these polysaccharides’ physicochemical and microstructural characteristics can be the starting point for elucidating their structure–function relationship as a valuable tool in advanced biomaterial design.
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