Oryeongsan通过NHE3信号通路调节SHR肾皮质RAS和NPS调节体液和Na+平衡

IF 2.8 4区 医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE
You Mee Ahn , Hye Yoom Kim , Dae Gill Kang , Kyung Woo Cho , Ho Sub Lee
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引用次数: 0

摘要

多灵山(ORS)已被用于治疗体液平衡受损。然而,所涉及的机制并没有明确界定。本研究旨在研究ORS对人类原发性高血压动物模型自发性高血压大鼠(SHR)肾脏排泄功能和血压(BP)的影响,并明确其与肾素-血管紧张素系统(RAS)和利钠肽系统(NPS)相关的机制。方法测定尿量(UV)、电解质(包括Na+排泄){尿中Na+排泄(UNaV)}的变化。RT-PCR检测大鼠肾皮质中RAS、NPS、钠(Na+)-氢(H+)交换剂3 (NHE3)的表达变化。结果与WKY-V组相比,shrv - v组SHR细胞的UV和UNaV均受到抑制,Na+平衡维持在较高水平,导致血压升高。这些都伴随着NHE3表达的增加,血管紧张素I转换酶-血管紧张素II型1 (ACE-AT1)受体的增强和肾皮质血管紧张素II型2 (AT2)受体/ACE2-Mas受体表达的同时抑制。与sbr - v相比,长期服用ORS治疗的sbr -ORS组的UV和UNaV增加,Na+和水平衡降低,血压降低。这些都伴随着NHE3表达的减少,ACE-AT1受体的抑制和AT2/ACE2-Mas受体的同时增强。结论ORS通过调节SHR中的RAS和NPS,抑制NHE3的表达,从而降低血压,降低Na+和水潴留。本研究为SHR患者口服补液治疗高血压提供了药理学依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Herbal medicine (Oryeongsan) for fluid and sodium balance in renal cortex of spontaneously hypertensive rats

Herbal medicine (Oryeongsan) for fluid and sodium balance in renal cortex of spontaneously hypertensive rats

Herbal medicine (Oryeongsan) for fluid and sodium balance in renal cortex of spontaneously hypertensive rats

Background

Herbal medicine Oryeongsan (ORS), also known as Wulingsan in Chinesehas been used for the treatment of impaired body fluid balance. However, the mechanisms involved are not clearly defined. The purpose of the present study was to identify the actions of ORS on the renal excretory function and blood pressure (BP) and to define the mechanisms involved in association with renin-angiotensin system (RAS) and natriuretic peptide system (NPS) in spontaneously hypertensive rats (SHR), an animal model of human essential hypertension.

Methods

Changes in urine volume (UV), excretion of electrolytes including Na+ (urinary excretion of Na+ (UNaV)) were measured. RT-PCR was performed to trace the changes in expression of RAS, NPS and sodium (Na+)-hydrogen (H+) exchanger 3 (NHE3) in the renal cortex.

Results

In the SHR treated with vehicle (SHR-V) group, UV and UNaV were suppressed and the Na+ balance was maintained at the higher levels leading to an increase in BP compared to WKY-V group. These were accompanied by an increase in NHE3 expression with an accentuation of angiotensin I converting enzyme-angiotensin II type 1 (ACE-AT1) receptor and concurrent suppression of angiotensin II type 2 (AT2) receptor/ACE2-Mas receptor expression in the renal cortex. Chronic treatment with ORS increased UV and UNaV, and decreased the Na+ and water balance with a decrease in BP in the ORS-treated SHR-ORS group compared to SHR-V. These were accompanied by a decrease in NHE3 expression with a suppression of ACE-AT1 receptor and concurrent accentuation of AT2/ACE2-Mas receptor.

Conclusion

The present study shows that ORS reduced BP with a decrease in Na+ and water retention by a suppression of NHE3 expression via modulation of RAS and NPS in SHR. The present study provides pharmacological rationale for the treatment of hypertension with ORS in SHR.

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来源期刊
Integrative Medicine Research
Integrative Medicine Research Medicine-Complementary and Alternative Medicine
CiteScore
6.50
自引率
2.90%
发文量
65
审稿时长
12 weeks
期刊介绍: Integrative Medicine Research (IMR) is a quarterly, peer-reviewed journal focused on scientific research for integrative medicine including traditional medicine (emphasis on acupuncture and herbal medicine), complementary and alternative medicine, and systems medicine. The journal includes papers on basic research, clinical research, methodology, theory, computational analysis and modelling, topical reviews, medical history, education and policy based on physiology, pathology, diagnosis and the systems approach in the field of integrative medicine.
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