TaEXPA19基因过表达提高冬小麦耐低温性

IF 1.5 4区 农林科学 Q2 AGRONOMY
Plant Breeding Pub Date : 2023-11-28 DOI:10.1111/pbr.13152
Fei Li, Baozhong Hu, Lina Peng, Xu Feng, Yu Miao, Jiamin Dong, Mingjing Wang, Xu Wang, Fenglan Li, Yongqing Xu
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引用次数: 0

摘要

低温是影响植物生长的主要非生物胁迫之一,可对植物造成严重伤害甚至死亡。TaEXPA19基因在冬小麦上、地下部分的表达差异及其对抗寒性的影响尚不清楚。本研究克隆了TaEXPA19基因,分析了TaEXPA19基因在冬小麦中的表达,构建了TaEXPA19基因转基因拟南芥,探讨了TaEXPA19基因在低温胁迫下对植物生长的影响。TaEXPA19-A和TaEXPA19-D基因在转基因拟南芥的上、地下部位有不同的应答模式。当植物受到低温胁迫时,叶片迅速上调,根系下调,然后上调以应对低温胁迫,促进叶片长度和叶宽、叶柄长度的生长。结果表明,TaEXPA19基因可以提高植物的低温耐受性。本研究结果为冬小麦抗寒性的研究奠定了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Overexpression of the TaEXPA19 gene improves low-temperature tolerance in winter wheat (Triticum aestivum)
Low temperature is one of the main abiotic stresses that affects plant growth, causing serious damage or even death to plants. The differential expression of the TaEXPA19 gene in the above and underground parts of winter wheat and the implications for cold resistance remain unclear. In this study, the TaEXPA19 gene was cloned and analysed for expression in winter wheat, and transgenic Arabidopsis thaliana was constructed to investigate the effect of the TaEXPA19 gene in response to low-temperature stress on plant growth. The TaEXPA19-A and TaEXPA19-D genes have different response patterns in the above and underground parts of transgenic A. thaliana. When plants were subjected to low-temperature stress, the leaves were quickly upregulated and the roots were downregulated, and then upregulated to respond to low-temperature stress to promote the growth of leaf length and leaf width petiole length. The results indicated that TaEXPA19 genes could improve low-temperature tolerance in plants. The results of this study laid a foundation for the study of the cold resistance of winter wheat.
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来源期刊
Plant Breeding
Plant Breeding 农林科学-农艺学
CiteScore
4.40
自引率
5.00%
发文量
74
审稿时长
3.0 months
期刊介绍: PLANT BREEDING publishes full-length original manuscripts and review articles on all aspects of plant improvement, breeding methodologies, and genetics to include qualitative and quantitative inheritance and genomics of major crop species. PLANT BREEDING provides readers with cutting-edge information on use of molecular techniques and genomics as they relate to improving gain from selection. Since its subject matter embraces all aspects of crop improvement, its content is sought after by both industry and academia. Fields of interest: Genetics of cultivated plants as well as research in practical plant breeding.
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