深圳1例致死性创伤弧菌的病原检测、回顾性分析及致病性分析。

IF 4.3 3区医学 Q1 GASTROENTEROLOGY & HEPATOLOGY

Gut Pathogens Pub Date : 2023-11-25 DOI:10.1186/s13099-023-00580-x

Shiqin Xu, Jinsong Wu, Ying Jin, Liyin Ji, Xuan Zou, Qinghua Hu, Tiejian Feng, Shuang Wu, Yixiang Jiang, Qiongcheng Chen, Huiqun Lu, Shuxiang Qiu, Huaisheng Chen, Min Jiang, Rui Cai, Yaqun Qiu, Xiaolu Shi

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引用次数: 0

摘要

我们报告一名36岁男性患者在餐厅食用牡蛎后死于创伤弧菌引起的败血症和多器官衰竭综合征。我们从患者血液样本中分离并鉴定出创伤弧菌vv16015，抗生素药敏试验显示对所有21种抗生素均敏感。随后从该餐厅的供应商处采集牡蛎样本，分离出三株创伤弧菌。全基因组测序和分析显示，vv16015与这些菌株有远亲关系，并证实该餐厅和供应商的海鲜中存在创伤弧菌污染。利用mellonella幼虫感染模型，确定了vv16015的毒力高于从存活患者(vv15018)和牡蛎(vv220015)分离的比较菌株。深圳市创伤弧菌在人群和环境中的分布具有散发性和异质性，与其他菌株相比，vv16015具有较高的毒力。

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Pathogenetic detection, retrospective and pathogenicity analysis of a fatal case of Vibrio vulnificus in Shenzhen, China.

We report a 36-year-old male patient died of V. vulnificus-induced septicaemia and multiple organ failure syndrome after oyster consumption at a restaurant. We isolated and identified V. vulnificus vv16015 from the patient's blood sample and antibiotic susceptibility tests indicated sensitivity to all 21 antibiotics. Oyster samples were subsequently collected from the restaurant's supplier and three strains of V. vulnificus were isolated. Whole genome sequencing and analysis revealed vv16015 to be distantly related to these strains and confirmed that V. vulnificus contamination was present in the seafood of the restaurant and supplier. Using a Galleria mellonella larvae infection model, the virulence of vv16015 was determined to be higher than that of comparison strains isolated from a surviving patient (vv15018) and an oyster (vv220015). The human and environment distribution of V. vulnificus in Shenzhen is sporadic and heterogeneous, and vv16015 is highly virulent compared to other strains.

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来源期刊

Gut Pathogens GASTROENTEROLOGY & HEPATOLOGY-MICROBIOLOGY

CiteScore

7.70

自引率

2.40%

发文量

期刊介绍： Gut Pathogens is a fast publishing, inclusive and prominent international journal which recognizes the need for a publishing platform uniquely tailored to reflect the full breadth of research in the biology and medicine of pathogens, commensals and functional microbiota of the gut. The journal publishes basic, clinical and cutting-edge research on all aspects of the above mentioned organisms including probiotic bacteria and yeasts and their products. The scope also covers the related ecology, molecular genetics, physiology and epidemiology of these microbes. The journal actively invites timely reports on the novel aspects of genomics, metagenomics, microbiota profiling and systems biology. Gut Pathogens will also consider, at the discretion of the editors, descriptive studies identifying a new genome sequence of a gut microbe or a series of related microbes (such as those obtained from new hosts, niches, settings, outbreaks and epidemics) and those obtained from single or multiple hosts at one or different time points (chronological evolution).