扩展抗php7抗体工具包:抗独特型纳米体抑制剂。

IF 3 Q3 IMMUNOLOGY

Antibodies Pub Date : 2023-11-16 DOI:10.3390/antib12040075

Susan K Vester, Anna M Davies, Rebecca L Beavil, Balraj S Sandhar, Andrew J Beavil, Hannah J Gould, Brian J Sutton, James M McDonnell

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引用次数: 0

摘要

我们之前已经生产了一个抗体工具包，包括所有的重组人抗体，除了一种人同型，直接针对polcalcin家族抗原php7。在这项工作中，我们用抗php7单克隆抗体的IgD版本完成了人抗体同型工具包。我们还提出了一组抗IgD抗php7抗体的纳米体，并鉴定和表征了一个paratope特异性纳米体。该纳米体还与具有相同独特型的抗体的IgE同型结合，并正畸抑制与phlp7的相互作用。描述了纳米体与IgD Fab配合物的2.1 Å分辨率x射线晶体结构。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Expanding the Anti-Phl p 7 Antibody Toolkit: An Anti-Idiotype Nanobody Inhibitor.

We have previously produced a toolkit of antibodies, comprising recombinant human antibodies of all but one of the human isotypes, directed against the polcalcin family antigen Phl p 7. In this work, we complete the toolkit of human antibody isotypes with the IgD version of the anti-Phl p 7 monoclonal antibody. We also raised a set of nanobodies against the IgD anti-Phl p 7 antibody and identify and characterize one paratope-specific nanobody. This nanobody also binds to the IgE isotype of this antibody, which shares the same idiotype, and orthosterically inhibits the interaction with Phl p 7. The 2.1 Å resolution X-ray crystal structure of the nanobody in complex with the IgD Fab is described.

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来源期刊

Antibodies IMMUNOLOGY-

CiteScore

7.10

自引率

6.40%

发文量

审稿时长

11 weeks

期刊介绍： Antibodies (ISSN 2073-4468), an international, peer-reviewed open access journal which provides an advanced forum for studies related to antibodies and antigens. It publishes reviews, research articles, communications and short notes. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. Full experimental and/or methodical details must be provided. Electronic files or software regarding the full details of the calculation and experimental procedure - if unable to be published in a normal way - can be deposited as supplementary material. This journal covers all topics related to antibodies and antigens, topics of interest include (but are not limited to): antibody-producing cells (including B cells), antibody structure and function, antibody-antigen interactions, Fc receptors, antibody manufacturing antibody engineering, antibody therapy, immunoassays, antibody diagnosis, tissue antigens, exogenous antigens, endogenous antigens, autoantigens, monoclonal antibodies, natural antibodies, humoral immune responses, immunoregulatory molecules.