[小肠结肠炎耶尔森菌——在含有鼠李糖、棉子糖和琥珀酸钠的营养培养基上的繁殖]。

Q4 Biochemistry, Genetics and Molecular Biology
Acta Microbiologica Bulgarica Pub Date : 1989-01-01
T Raducheva, D Velianov, L Ilieva
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引用次数: 0

摘要

研究了小肠结肠炎耶尔森菌血清型0:3、0:5、0:8和0:9在鼠李糖、棉子糖和琥珀酸钠培养基上不同培养温度(4℃、25℃和37℃)下的体外生长动力学。结果表明,在25℃培养的所有小肠结肠炎耶尔森菌血清型均具有最高的生长速率。当l -鼠李糖,特别是琥珀酸钠作为底物时,在这个温度下观察到显著的生长激活。37℃的培养使其生长速度急剧增加,但在实验结束时(培养第96小时)生长速度下降,并且在含d -棉子糖的培养基上观察到一定的生长刺激。以l -鼠李糖为底物的细胞在4℃培养24小时后开始生长,血清型0:8和0:9表达较好。本研究结果可用于不同来源小肠结肠炎耶尔森菌在含l -鼠李糖培养基上4℃培养后的分离实验。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Yersinia enterocolitica--its multiplication on nutrient media containing rhamnose, raffinose and sodium succinate].

The kinetics of growth in vitro of strains Yersinia enterocolitica, serovars 0:3, 0:5, 0:8 and 0:9, on culture media containing rhamnose, raffinose and sodium succinate and at different temperatures of cultivation (4 degrees, 25 degrees and 37 degrees C) was characterized. It was established, that all the Yersinia enterocolitica serovars tested, cultivated at 25 degrees C, had the highest growth rate. A significant activation of growth at this temperature was observed when L-rhamnose and especially sodium succinate were used as substrates. The cultivation at 37 degrees C caused a sharp increase in the growth rate which, however, decreased at the end of the experiment (96th hour of cultivation), and certain stimulation of growth was observed on D-raffinose-containing medium. The growth of cells cultivated at 4 degrees C using L-rhamnose as substrate started after a lag-phase of 24 hours, which was better expressed for serovars 0:8 and 0:9. The results reported could be applied to experiments, using methods of isolation of Yersinia enterocolitica from different sources after cultivation at 4 degrees C on L-rhamnose-containing media.

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来源期刊
Acta Microbiologica Bulgarica
Acta Microbiologica Bulgarica Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
0.40
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