Shun Lu , Wenjing Ye , Jie Zhou , Guangming Yi , Jianming Huang , Siyao Deng , Minglun Li , Yimin Li , Yanqiong Song , Jiayu Zhang , Lichun Wei , Guiquan Zhu , Jinyi Lang
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In addition, SA increased radiation-induced DNA damage, promoted apoptosis, triggered a redistribution of cell cycle from G<sub>2</sub>-M phase to G<sub>1</sub>-S phase of cervical cancer cells, and hence increased cell sensitivity to radiation. Moreover, SA treatment elevated the expression levels of p-AMPKα(<em>t</em> = 3.996, <em>P</em> < 0.05) and p-TSC2(<em>t</em> = 5.308, <em>P</em> < 0.05), whereas the level of p-mTOR (<em>t</em> = 10.160, <em>P</em> < 0.05) was significantly decreased.</p></div><div><h3>Conclusion</h3><p>SA enhances the radiosensitivity of cervical cancer cells by targeting AMPK/TSC2/mTOR signaling pathway, and might serve as a promising therapeutic strategy to improve the efficacy of radiotherapy for cervical cancer.</p></div>","PeriodicalId":34051,"journal":{"name":"Radiation Medicine and Protection","volume":"3 1","pages":"Pages 9-15"},"PeriodicalIF":0.0000,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666555722000053/pdfft?md5=69bdc019a46d70a17e2b4dd2f5d5c2c0&pid=1-s2.0-S2666555722000053-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Salicylic acid sensitizes cervical cancer cells to radiotherapy by activating AMPK/TSC2/mTOR pathway\",\"authors\":\"Shun Lu , Wenjing Ye , Jie Zhou , Guangming Yi , Jianming Huang , Siyao Deng , Minglun Li , Yimin Li , Yanqiong Song , Jiayu Zhang , Lichun Wei , Guiquan Zhu , Jinyi Lang\",\"doi\":\"10.1016/j.radmp.2022.01.005\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objective</h3><p>To investigate the radio-sensitizing effect of salicylic acid (SA) on human cervical cancer cells and its potential molecular mechanism.</p></div><div><h3>Methods</h3><p>Cervical cancer cells were treated with SA and ionizing radiation. The expression of γ-H2AX was evaluated by immunofluorescence (IF) assay. Cell cycle and apoptosis were analyzed by flow cytometry. Western blot was performed to detect the protein level of AMPK/TSC2/mTOR pathway.</p></div><div><h3>Results</h3><p>SA inhibited basal proliferation of cervical cancer cells in a dose and time dependent manner. In addition, SA increased radiation-induced DNA damage, promoted apoptosis, triggered a redistribution of cell cycle from G<sub>2</sub>-M phase to G<sub>1</sub>-S phase of cervical cancer cells, and hence increased cell sensitivity to radiation. 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引用次数: 0
摘要
目的探讨水杨酸(SA)对人宫颈癌细胞的放射增敏作用及其可能的分子机制。方法分别用SA和电离辐射处理宫颈癌细胞。免疫荧光法检测γ-H2AX的表达。流式细胞术分析细胞周期和凋亡情况。Western blot检测AMPK/TSC2/mTOR通路蛋白表达水平。结果sa对宫颈癌细胞基底增殖的抑制作用呈剂量依赖性和时间依赖性。此外，SA增加了辐射诱导的DNA损伤，促进了细胞凋亡，引发宫颈癌细胞周期由G2-M期向G1-S期重新分布，从而增加了细胞对辐射的敏感性。此外，SA处理提高了P - ampk α的表达水平(t = 3.996, P <0.05)和P - tsc2 (t = 5.308, P <0.05),而p-mTOR水平(t = 10.160, P & lt;0.05)显著降低。结论sa通过靶向AMPK/TSC2/mTOR信号通路增强宫颈癌细胞的放射敏感性，可能是提高宫颈癌放疗疗效的一种有前景的治疗策略。
Salicylic acid sensitizes cervical cancer cells to radiotherapy by activating AMPK/TSC2/mTOR pathway
Objective
To investigate the radio-sensitizing effect of salicylic acid (SA) on human cervical cancer cells and its potential molecular mechanism.
Methods
Cervical cancer cells were treated with SA and ionizing radiation. The expression of γ-H2AX was evaluated by immunofluorescence (IF) assay. Cell cycle and apoptosis were analyzed by flow cytometry. Western blot was performed to detect the protein level of AMPK/TSC2/mTOR pathway.
Results
SA inhibited basal proliferation of cervical cancer cells in a dose and time dependent manner. In addition, SA increased radiation-induced DNA damage, promoted apoptosis, triggered a redistribution of cell cycle from G2-M phase to G1-S phase of cervical cancer cells, and hence increased cell sensitivity to radiation. Moreover, SA treatment elevated the expression levels of p-AMPKα(t = 3.996, P < 0.05) and p-TSC2(t = 5.308, P < 0.05), whereas the level of p-mTOR (t = 10.160, P < 0.05) was significantly decreased.
Conclusion
SA enhances the radiosensitivity of cervical cancer cells by targeting AMPK/TSC2/mTOR signaling pathway, and might serve as a promising therapeutic strategy to improve the efficacy of radiotherapy for cervical cancer.
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