{"title":"钝头涡虫多巴胺敏感腺苷酸环化酶的研究","authors":"Giorgio Venturini","doi":"10.1016/0742-8413(93)90211-3","DOIUrl":null,"url":null,"abstract":"<div><p>1. The adenylate cyclase activity present in the particulate fraction of planaria homogenates has been characterized.</p><p>2. The enzyme requires divalent cations (Mg<sup>2+</sup>), and a <em>K</em><sub>m</sub> for ATP of 0.58 at 30°C was measured.</p><p>3. GTP and Gpp(NH)p, in an optimal range of 10<sup>−4</sup>–10<sup>−5</sup>M, increase the enzymatic activity.</p><p>4. In the presence of GTP, dopamine stimulates the adenylate cyclase and its action is inhibited by dopaminergic antagonist.</p><p>5. Both D-1 and D-2 selective dopaminergic agonists stimulate the enzymatic activity and their action is selectively antagonized by D-1 and D-2 antagonists.</p><p>6. The high concentrations required for some D-1 and D-2 agents to be effective, suggest an only partial consistency with mammalian dopaminergic receptors.</p></div>","PeriodicalId":72650,"journal":{"name":"Comparative biochemistry and physiology. C: Comparative pharmacology","volume":"105 2","pages":"Pages 297-301"},"PeriodicalIF":0.0000,"publicationDate":"1993-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0742-8413(93)90211-3","citationCount":"1","resultStr":"{\"title\":\"Dopamine sensitive adenylate cyclase in planaria Dugesia gonocephala\",\"authors\":\"Giorgio Venturini\",\"doi\":\"10.1016/0742-8413(93)90211-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>1. The adenylate cyclase activity present in the particulate fraction of planaria homogenates has been characterized.</p><p>2. The enzyme requires divalent cations (Mg<sup>2+</sup>), and a <em>K</em><sub>m</sub> for ATP of 0.58 at 30°C was measured.</p><p>3. GTP and Gpp(NH)p, in an optimal range of 10<sup>−4</sup>–10<sup>−5</sup>M, increase the enzymatic activity.</p><p>4. In the presence of GTP, dopamine stimulates the adenylate cyclase and its action is inhibited by dopaminergic antagonist.</p><p>5. Both D-1 and D-2 selective dopaminergic agonists stimulate the enzymatic activity and their action is selectively antagonized by D-1 and D-2 antagonists.</p><p>6. The high concentrations required for some D-1 and D-2 agents to be effective, suggest an only partial consistency with mammalian dopaminergic receptors.</p></div>\",\"PeriodicalId\":72650,\"journal\":{\"name\":\"Comparative biochemistry and physiology. C: Comparative pharmacology\",\"volume\":\"105 2\",\"pages\":\"Pages 297-301\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1993-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0742-8413(93)90211-3\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Comparative biochemistry and physiology. C: Comparative pharmacology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0742841393902113\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Comparative biochemistry and physiology. C: Comparative pharmacology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0742841393902113","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Dopamine sensitive adenylate cyclase in planaria Dugesia gonocephala
1. The adenylate cyclase activity present in the particulate fraction of planaria homogenates has been characterized.
2. The enzyme requires divalent cations (Mg2+), and a Km for ATP of 0.58 at 30°C was measured.
3. GTP and Gpp(NH)p, in an optimal range of 10−4–10−5M, increase the enzymatic activity.
4. In the presence of GTP, dopamine stimulates the adenylate cyclase and its action is inhibited by dopaminergic antagonist.
5. Both D-1 and D-2 selective dopaminergic agonists stimulate the enzymatic activity and their action is selectively antagonized by D-1 and D-2 antagonists.
6. The high concentrations required for some D-1 and D-2 agents to be effective, suggest an only partial consistency with mammalian dopaminergic receptors.
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