多组学分析揭示了植物乳杆菌预防lps诱导蛋鸡输卵管炎的分子调控网络。

IF 6.3 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Dan Song, Aike Li, Bingxu Chen, Jia Feng, Tao Duan, Junlin Cheng, Lixian Chen, Weiwei Wang, Yuna Min
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引用次数: 0

摘要

背景:输卵管炎是蛋鸡生产中的常见疾病之一,严重影响蛋鸡养殖的经济效益。植物乳杆菌可有效预防局部或全身炎症,但其预防输卵管炎的研究报道甚少。本研究旨在通过多组学分析,包括微生物组学、转录组学和代谢组学分析,探讨微囊化植物乳杆菌(MLP)对输卵管炎的预防分子调控网络。结果:结果显示,饲粮中添加MLP可显著减轻蛋鸡脂多糖(LPS)引起的子宫炎症和子宫萎缩(P)。综上所述,饲粮中添加微囊化植物乳杆菌可通过调节Candidatus_Saccharimonas、Phascolarctobacterium、Ruminococcus_torques_group和Eubacterium_hallii_group的丰度,下调血浆代谢物、对巯基硫酸盐、o-甲酚和n -乙酰组胺的水平,上调s -乳酰谷胱甘肽的水平,同时增加子宫内CPNE4、CNTN3和ACAN基因的表达,从而预防输卵管炎。并最终抑制输卵管炎症。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Multi-omics analysis reveals the molecular regulatory network underlying the prevention of Lactiplantibacillus plantarum against LPS-induced salpingitis in laying hens.

Background: Salpingitis is one of the common diseases in laying hen production, which greatly decreases the economic outcome of laying hen farming. Lactiplantibacillus plantarum was effective in preventing local or systemic inflammation, however rare studies were reported on its prevention against salpingitis. This study aimed to investigate the preventive molecular regulatory network of microencapsulated Lactiplantibacillus plantarum (MLP) against salpingitis through multi-omics analysis, including microbiome, transcriptome and metabolome analyses.

Results: The results revealed that supplementation of MLP in diet significantly alleviated the inflammation and atrophy of uterus caused by lipopolysaccharide (LPS) in hens (P < 0.05). The concentrations of plasma IL-2 and IL-10 in hens of MLP-LPS group were higher than those in hens of LPS-stimulation group (CN-LPS group) (P < 0.05). The expression levels of TLR2, MYD88, NF-κB, COX2, and TNF-α were significantly decreased in the hens fed diet supplemented with MLP and suffered with LPS stimulation (MLP-LPS group) compared with those in the hens of CN-LPS group (P < 0.05). Differentially expressed genes (DEGs) induced by  MLP were involved in inflammation, reproduction, and calcium ion transport. At the genus level, the MLP supplementation significantly increased  the abundance of Phascolarctobacterium, whereas decreased the abundance of Candidatus_Saccharimonas in LPS challenged hens (P < 0.05). The metabolites altered by dietary supplementation with MLP were mainly involved in galactose, uronic acid, histidine, pyruvate and primary bile acid metabolism. Dietary supplementation with MLP inversely regulates LPS-induced differential metabolites such as LysoPA (24:0/0:0) (P < 0.05).

Conclusions: In summary, dietary supplementation with microencapsulated Lactiplantibacillus plantarum prevented salpingitis by modulating the abundances of Candidatus_Saccharimonas, Phascolarctobacterium, Ruminococcus_torques_group and Eubacterium_hallii_group while downregulating the levels of plasma metabolites, p-tolyl sulfate, o-cresol and N-acetylhistamine and upregulating S-lactoylglutathione, simultaneously increasing the expressions of CPNE4, CNTN3 and ACAN genes in the uterus, and ultimately inhibiting oviducal inflammation.

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