Débora Ferreira dos Santos Angelo, Vanessa Brito de Souza Rabello, Maria Adrielle Soares Maciel, Sheilla Suellenn de Lemos Araújo Atanázio, Maria Clara Lima da Costa, Suely Ruth Silva, Rodrigo Almeida-Paes, Andréa Reis Bernardes-Engemann, Rosely Maria Zancopé-Oliveira, Inácio José Clementino
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Thirty-nine samples were analyzed, obtained from skin lesions of domestic felines, from the following cities in Paraíba: João Pessoa, Pilões, Patos, Areia, Bananeiras and Guarabira. Cytological analysis was performed to screen the samples, followed by fungal culture, and the molecular characterization of the isolates was performed, using the species-specific Polymerase Chain Reaction (PCR) or partial sequencing of the calmodulin gene. All isolates were identified as S. brasiliensis. The sequencing showed 100% similarity to the S. brasiliensis CBS 120339 strain. In view of this, it is concluded that in the study areas the species involved in cases of feline sporotrichosis is S. brasiliensis, its presence in Paraíba demonstrated the spread of the agent in regions distant from the epicenters in Brazil, alerting to the possible occurrence of zoonotic outbreaks similar to those found in the South and Southeast regions of the country. 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引用次数: 0
摘要
摘要:孢子菌病是一种由孢子菌群真菌引起的皮下真菌病,在巴西报道的主要菌种是巴西孢子菌,病猫是其传播者。尽管自2016年以来,其在巴西Paraíba州的发病率有所增加,但该州有关该疾病的数据有限。因此,本研究旨在鉴定Paraíba城市家猫孢子丝菌的分子分离株,从而扩大对该州该病的认识。对来自Paraíba以下城市的39个家猫皮肤病变样本进行了分析:jo o Pessoa、Pilões、Patos、Areia、Bananeiras和Guarabira。对样品进行细胞学分析筛选,然后进行真菌培养,并使用物种特异性聚合酶链反应(PCR)或钙调素基因的部分测序对分离物进行分子表征。所有分离株均鉴定为巴西葡萄球菌。测序结果显示,该菌株与巴西孢子虫CBS 120339株的相似性为100%。鉴于此,我们得出结论,在研究地区的猫孢子虫病病例所涉及的物种是巴西孢子虫,它在Paraíba的存在表明该媒介在远离巴西疫源地的地区传播,提示可能发生与该国南部和东南部地区类似的人畜共患疾病暴发。此外,报告还强调了猫科动物在巴西新流行地区孢子虫病传播中的新作用。
Sporothrix brasiliensis infecting cats in northeastern Brazil: New emerging areas in Paraíba state
ABSTRACT: Sporotrichosis is a subcutaneous mycosis caused by fungus of the Sporothrix complex, and in Brazil the main species reported is Sporothrix brasiliensis, of which the diseased cat is the transmitter. Although, its occurrence has increased in the state of Paraíba, Brazil, since 2016, data on the disease in this state are limited. Therefore, this research aimed to identify molecularly isolates of Sporothrix spp. from domestic cats from cities in Paraíba, and in this way to expand the understanding of the disease in the state. Thirty-nine samples were analyzed, obtained from skin lesions of domestic felines, from the following cities in Paraíba: João Pessoa, Pilões, Patos, Areia, Bananeiras and Guarabira. Cytological analysis was performed to screen the samples, followed by fungal culture, and the molecular characterization of the isolates was performed, using the species-specific Polymerase Chain Reaction (PCR) or partial sequencing of the calmodulin gene. All isolates were identified as S. brasiliensis. The sequencing showed 100% similarity to the S. brasiliensis CBS 120339 strain. In view of this, it is concluded that in the study areas the species involved in cases of feline sporotrichosis is S. brasiliensis, its presence in Paraíba demonstrated the spread of the agent in regions distant from the epicenters in Brazil, alerting to the possible occurrence of zoonotic outbreaks similar to those found in the South and Southeast regions of the country. In addition, it highlights the emerging role of felines in the transmission of sporotrichosis in new endemic areas of Brazil.
期刊介绍:
The purpose of Ciência Rural is to publish the results of original research, note and reviews which contribute significantly to knowledge in Agricultural Sciences. Preference will be given to original articles that develop news concepts or experimental approaches and are not merely repositories of scientific data. The decison of acceptance for publication lies with the Editors and is based on the recommendations of Editorial Comission, Area Committee and/ or ad hoc reviewers. The editors and reviewers are external to the institution.